Hot 100 number-one hits of 2001 (United States)
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This is a list of the U.S. Billboard magazine Hot 100 number-ones of 2001.
| Issue Date | Song | Artist |
| January 6 | "Independent Women Part I" | Destiny's Child |
| January 13 | "Independent Women Part I" | Destiny's Child |
| January 20 | "Independent Women Part I" | Destiny's Child |
| January 27 | "Independent Women Part I" | Destiny's Child |
| February 3 | "It Wasn't Me" | Shaggy featuring Ricardo "RikRok" Ducent |
| February 10 | "It Wasn't Me" | Shaggy featuring Ricardo "RikRok" Ducent |
| February 17 | "Ms. Jackson" | OutKast |
| February 24 | "Stutter" | Joe featuring Mystikal |
| March 3 | "Stutter" | Joe featuring Mystikal |
| March 10 | "Stutter" | Joe featuring Mystikal |
| March 17 | "Stutter" | Joe featuring Mystikal |
| March 24 | "Butterfly" | Crazy Town |
| March 31 | "Angel" | Shaggy featuring Rayvon |
| April 7 | "Butterfly" | Crazy Town |
| April 14 | "All for You" | Janet |
| April 21 | "All for You" | Janet |
| April 28 | "All for You" | Janet |
| May 5 | "All for You" | Janet |
| May 12 | "All for You" | Janet |
| May 19 | "All for You" | Janet |
| May 26 | "All for You" | Janet |
| June 2 | "Lady Marmalade" | Christina Aguilera, Lil' Kim, Mýa and Pink |
| June 9 | "Lady Marmalade" | Christina Aguilera, Lil' Kim, Mýa and Pink |
| June 16 | "Lady Marmalade" | Christina Aguilera, Lil' Kim, Mýa and Pink |
| June 23 | "Lady Marmalade" | Christina Aguilera, Lil' Kim, Mýa and Pink |
| June 30 | "Lady Marmalade" | Christina Aguilera, Lil' Kim, Mýa and Pink |
| July 7 | "U Remind Me" | Usher |
| July 14 | "U Remind Me" | Usher |
| July 21 | "U Remind Me" | Usher |
| July 28 | "U Remind Me" | Usher |
| August 4 | "Bootylicious" | Destiny's Child |
| August 11 | "Bootylicious" | Destiny's Child |
| August 18 | "Fallin'" | Alicia Keys |
| August 25 | "Fallin'" | Alicia Keys |
| September 1 | "Fallin'" | Alicia Keys |
| September 8 | "I'm Real" | Jennifer Lopez featuring Ja Rule |
| September 15 | "I'm Real" | Jennifer Lopez featuring Ja Rule |
| September 22 | "I'm Real" | Jennifer Lopez featuring Ja Rule |
| September 29 | "Fallin'" | Alicia Keys |
| October 6 | "Fallin'" | Alicia Keys |
| October 13 | "Fallin'" | Alicia Keys |
| October 20 | "I'm Real" | Jennifer Lopez featuring Ja Rule |
| October 27 | "I'm Real" | Jennifer Lopez featuring Ja Rule |
| November 3 | "Family Affair" | Mary J. Blige |
| November 10 | "Family Affair" | Mary J. Blige |
| November 17 | "Family Affair" | Mary J. Blige |
| November 24 | "Family Affair" | Mary J. Blige |
| December 1 | "Family Affair" | Mary J. Blige |
| December 8 | "Family Affair" | Mary J. Blige |
| December 15 | "U Got It Bad" | Usher |
| December 22 | "How You Remind Me" | Nickelback |
| December 29 | "How You Remind Me" | Nickelback |
