Hot 100 number-one hits of 2000 (United States)
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This is a list of the U.S. Billboard magazine Hot 100 number-ones of 2000.
| Issue Date | Song | Artist |
| January 1 | "Smooth" | Santana featuring Rob Thomas |
| January 8 | "Smooth" | Santana featuring Rob Thomas |
| January 15 | "What a Girl Wants" | Christina Aguilera |
| January 22 | "What a Girl Wants" | Christina Aguilera |
| January 29 | "I Knew I Loved You" | Savage Garden |
| February 5 | "I Knew I Loved You" | Savage Garden |
| February 12 | "I Knew I Loved You" | Savage Garden |
| February 19 | "Thank God I Found You" | Mariah Carey featuring Joe and 98 Degrees |
| February 26 | "I Knew I Loved You" | Savage Garden |
| March 4 | "Amazed" | Lonestar |
| March 11 | "Amazed" | Lonestar |
| March 18 | "Say My Name" | Destiny's Child |
| March 25 | "Say My Name" | Destiny's Child |
| April 1 | "Say My Name" | Destiny's Child |
| April 8 | "Maria Maria" | Santana featuring The Product G&B |
| April 15 | "Maria Maria" | Santana featuring The Product G&B |
| April 22 | "Maria Maria" | Santana featuring The Product G&B |
| April 29 | "Maria Maria" | Santana featuring The Product G&B |
| May 6 | "Maria Maria" | Santana featuring The Product G&B |
| May 13 | "Maria Maria" | Santana featuring The Product G&B |
| May 20 | "Maria Maria" | Santana featuring The Product G&B |
| May 27 | "Maria Maria" | Santana featuring The Product G&B |
| June 3 | "Maria Maria" | Santana featuring The Product G&B |
| June 10 | "Maria Maria" | Santana featuring The Product G&B |
| June 17 | "Try Again" | Aaliyah |
| June 24 | "Be with You" | Enrique Iglesias |
| July 1 | "Be with You" | Enrique Iglesias |
| July 8 | "Be with You" | Enrique Iglesias |
| July 15 | "Everything You Want" | Vertical Horizon |
| July 22 | "Bent" | Matchbox Twenty |
| July 29 | "It's Gonna Be Me" | 'N Sync |
| August 5 | "It's Gonna Be Me" | 'N Sync |
| August 12 | "Incomplete" | Sisqó |
| August 19 | "Incomplete" | Sisqó |
| August 26 | "Doesn't Really Matter" | Janet |
| September 2 | "Doesn't Really Matter" | Janet |
| September 9 | "Doesn't Really Matter" | Janet |
| September 16 | "Music" | Madonna |
| September 23 | "Music" | Madonna |
| September 30 | "Music" | Madonna |
| October 7 | "Music" | Madonna |
| October 14 | "Come on Over Baby (All I Want Is You)" | Christina Aguilera |
| October 21 | "Come on Over Baby (All I Want Is You)" | Christina Aguilera |
| October 28 | "Come on Over Baby (All I Want Is You)" | Christina Aguilera |
| November 4 | "Come on Over Baby (All I Want Is You)" | Christina Aguilera |
| November 11 | "With Arms Wide Open" | Creed |
| November 18 | "Independent Women Part I" | Destiny's Child |
| November 25 | "Independent Women Part I" | Destiny's Child |
| December 2 | "Independent Women Part I" | Destiny's Child |
| December 9 | "Independent Women Part I" | Destiny's Child |
| December 16 | "Independent Women Part I" | Destiny's Child |
| December 23 | "Independent Women Part I" | Destiny's Child |
| December 30 | "Independent Women Part I" | Destiny's Child |
