Plant transformation vector

From Wikipedia, the free encyclopedia

 This article needs additional citations for verification.
Please help improve this article by adding reliable references. Unsourced material may be challenged and removed. (July 2007)

Contents

[edit] Plant transformation vectors

Plant transformation vectors are plasmids that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used plant transformation vectors are termed binary vectors because of their ability to replicate in both E. coli and Agrobacterium tumefaciens. Plant Transformation vectors contain three key elements;

¤ Plasmids Selection

¤ Plasmids Replication

¤ T-DNA region

[edit] Plasmid Selection

In order for a plasmid to be distinguished from bacterial cells that do not contain a binary vector, a plasmid selection genes is needed. In many cases this is a resistance to antibiotic such as kanamycin, ampicillin, spectinomycin or tetracyclin. This antibiotic gene is not usually transferred to the plant cell but remains within the bacterial cell

[edit] Plasmids Replication

Plasmids replicate to produce many plasmid molecules in each bacterial cell. The number of copies of each plasmid in a bacterial cell is determined by the replication origin. This is the position within the plasmids molecule where DNA replication is initiated. Most binary vectors have a higher number of plasmids copies when they replicate in E. coli, the plasmid copy-number is usually less when the plasmid resident within Agrobacterium tumefaciens.

[edit] T-DNA region

[edit] External links