Chloramphenicol acetyltransferase

From Wikipedia, the free encyclopedia

Ribbon diagram of the chloramphenicol acetyltransferase trimer with chloramphenicol bound. From PDB 3CLA.
chloramphenicol acetyltransferase
Identifiers
Symbol	CAT
UniProt	P62577
Other data
EC number	2.3.1.28

Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme that detoxifies the antibiotic chloramphenicol. It is responsible for chloramphenicol resistance in bacteria. This enzyme covalently attaches an acetyl group from acetyl-CoA to the chloramphenicol molecule so that it is unable to bind to the ribosome. CAT was used as a reporter system to measure the level of a promoter or its tissue-specific expression. The CAT assay involves monitoring acetylation of radioactive chloramphenicol on a TCA plate because acetylation significantly changes migration rate.

v • d • e Transferases: acyltransferases (EC 2.3) 2.3.1: other than amino-acyl groups (mostly acetyltransferases) N-Acetylglutamate synthase - Choline acetyltransferase - Acetyl-Coenzyme A acetyltransferase - Dihydrolipoyl transacetylase - Acetyl-CoA C-acyltransferase - Beta-galactoside transacetylase - Carnitine O-palmitoyltransferase (CPT1, CPT2) - Acyltransferase like 2 - Chloramphenicol acetyltransferase - Aminolevulinic acid synthase - Beta-ketoacyl-ACP synthase - Glyceronephosphate O-acyltransferase - Lecithin-cholesterol acyltransferase - Histone acetyltransferase (P300/CBP) - Serotonin N-acetyl transferase - Serine C-palmitoyltransferase (SPTLC1, SPTLC2) - HGSNAT 2.3.2 - Aminoacyltransferases Gamma-glutamyl transpeptidase - Peptidyl transferase - Transglutaminase (Tissue transglutaminase, Keratinocyte transglutaminase, Factor XIII) 2.3.3 - converted into alkyl on transfer Citrate synthase - ATP citrate lyase - HMG-CoA synthase