6-methylsalicylic-acid synthase

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In enzymology, a 6-methylsalicylic-acid synthase (EC 2.3.1.165) is an enzyme that catalyzes the chemical reaction

acetyl-CoA + 3 malonyl-CoA + NADPH + H⁺ $\rightleftharpoons$ 6-methylsalicylate + 4 CoA + 3 CO₂ + NADP⁺ + H₂O

The 4 substrates of this enzyme are acetyl-CoA, malonyl-CoA, NADPH, and H⁺, whereas its 5 products are 6-methylsalicylate, CoA, CO₂, NADP⁺, and H₂O.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:malonyl-CoA C-acyltransferase (decarboxylating, oxoacyl-reducing, thioester-hydrolysing and cyclizing). Other names in common use include MSAS, and 6-methylsalicylic acid synthase.

[edit] References

IUBMB entry for 2.3.1.165
BRENDA references for 2.3.1.165 (Recommended.)
PubMed references for 2.3.1.165
PubMed Central references for 2.3.1.165
Google Scholar references for 2.3.1.165
Spencer JB, Jordan PM (Pt 3). "Purification and properties of 6-methylsalicylic acid synthase from Penicillium patulum". Biochem. J. 288: 839–46. PMID 1471999.
Child CJ, Spencer JB, Bhogal P, Shoolingin-Jordan PM (1996). "Structural similarities between 6-methylsalicylic acid synthase from Penicillium patulum and vertebrate type I fatty acid synthase: evidence from thiol modification studies". Biochemistry. 35: 12267–74. PMID 8823160.
Richardson MT, Pohl NL, Kealey JT, Khosla C (1999). "Tolerance and specificity of recombinant 6-methylsalicyclic acid synthase". Metab. Eng. 1: 180–7. PMID 10935930.