Talk:Southern blot
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Article Grading: The following comments were left by the quality and importance raters: (edit · history · refresh · how to use this template) The description of the hybridization probe seems a little incorrect to me. The probe is often made by "Klenow labeling" and random hexamer/octamer primers are used. The template is usually a plasmid fragment/PCR product thus usually two DNA strands are available as templates in the reaction. So the actual probe is a mixture of fragments - not a single specific fragment. Moreover, the probe binds to a complementary sequence, and not one identical to it. I had a feeling this was not too clear from the article.
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[edit] Transfer of DNA fragments onto nitrocelluose paper
You've skirted over the issue a bit. To my understanding using the (older) capillary method filter paper is used to wick buffer so that it flows through the gel and carries the DNA fragments to the membrane. Effectively blotting the DNA bands from the gel to the nitrocellulose paper. Apparently nowadays Electrolytic transfer is often used as well/ instead.
[edit] Hawaiian Blot?
I cannot recall a Hawaiian blot and it has no link, what is this method for detecting biochemicals? SableSynthesis 15:08, 28 March 2006 (UTC)
[edit] request for information
This was moved from the article JetheroTalk 03:49, 22 April 2007 (UTC)
We should add some info so kids doing their homework can get the answers
[edit] Capitalization of other blots
The CDC page on writing style says that capitalized Western blot is the preferred. I'm sure one can make arguments for or against capitalizing, but usually style should conform to one standard and the CDC is a good enough authority. Calgirl2 (talk) 18:23, 29 November 2007 (UTC)
- I think that you will need to discuss this on Western blot, because this entry uses likewise "western blot" rather than the capitalized form, and so style within Wikipedia would be inconsistent. But I'd avoid flogging dead horses, as camps pro and con capitalization are firmly entrenched, and both have good arguments for making their cases. Malljaja (talk) 19:14, 29 November 2007 (UTC)
- A brief addition to my comment. I note that the CDC lists Southern and Western blot as requiring capitals, but not their methodological cousin, northern blot--this is inconsistent and may may rest on CDC's erroneous assumption (which I've heard before) that the western blot was invented by someone called "Western". Malljaja (talk) 19:20, 29 November 2007 (UTC)
