Sirohydrochlorin cobaltochelatase

From Wikipedia, the free encyclopedia

In enzymology, a sirohydrochlorin cobaltochelatase (EC 4.99.1.3) is an enzyme that catalyzes the chemical reaction

cobalt-sirohydrochlorin + 2 H⁺ $\rightleftharpoons$ sirohydrochlorin + Co₂+

Thus, the two substrates of this enzyme are cobalt-sirohydrochlorin and H⁺, whereas its two products are sirohydrochlorin and Co2+.

This enzyme belongs to the family of lyases, specifically the "catch-all" class of lyases that do not fit into any other sub-class. The systematic name of this enzyme class is cobalt-sirohydrochlorin cobalt-lyase (sirohydrochlorin-forming). Other names in common use include CbiK, CbiX, CbiXS, anaerobic cobalt chelatase, cobaltochelatase [ambiguous], and sirohydrochlorin cobalt-lyase (incorrect). This enzyme participates in porphyrin and chlorophyll metabolism.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1TJN and 2DJ5.

[edit] References

IUBMB entry for 4.99.1.3
BRENDA references for 4.99.1.3 (Recommended.)
PubMed references for 4.99.1.3
PubMed Central references for 4.99.1.3
Google Scholar references for 4.99.1.3
Schubert HL, Raux E, Wilson KS, Warren MJ (1999). "Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis". Biochemistry. 38: 10660–9. doi:10.1021/bi9906773. PMID 10451360.
Brindley AA, Raux E, Leech HK, Schubert HL, Warren MJ (2003). "A story of chelatase evolution: identification and characterization of a small 13-15-kDa "ancestral" cobaltochelatase (CbiXS) in the archaea". J. Biol. Chem. 278: 22388–95. doi:10.1074/jbc.M302468200. PMID 12686546.
Warren MJ, Raux E, Schubert HL, Escalante-Semerena JC (2002). "The biosynthesis of adenosylcobalamin (vitamin B12)". Nat. Prod. Rep. 19: 390–412. doi:10.1039/b108967f. PMID 12195810.