S-(hydroxymethyl)glutathione dehydrogenase

From Wikipedia, the free encyclopedia

In enzymology, a S-(hydroxymethyl)glutathione dehydrogenase (EC 1.1.1.284) is an enzyme that catalyzes the chemical reaction

S-(hydroxymethyl)glutathione + NAD(P)+ $\rightleftharpoons$ S-formylglutathione + NAD(P)H + H⁺

The 3 substrates of this enzyme are S-(hydroxymethyl)glutathione, NAD⁺, and NADP⁺, whereas its 4 products are S-formylglutathione, NADH, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is S-(hydroxymethyl)glutathione:NAD+ oxidoreductase. Other names in common use include NAD-linked formaldehyde dehydrogenase (incorrect), formaldehyde dehydrogenase (incorrect), formic dehydrogenase (incorrect), class III alcohol dehydrogenase, ADH3, &chi, -ADH, FDH (incorrect), formaldehyde dehydrogenase (glutathione) (incorrect), GS-FDH (incorrect), glutathione-dependent formaldehyde dehydrogenase (incorrect), NAD-dependent formaldehyde dehydrogenase, GD-FALDH, and NAD- and glutathione-dependent formaldehyde dehydrogenase. This enzyme participates in methane metabolism.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 2FZE and 2FZW.

[edit] References

IUBMB entry for 1.1.1.284
BRENDA references for 1.1.1.284 (Recommended.)
PubMed references for 1.1.1.284
PubMed Central references for 1.1.1.284
Google Scholar references for 1.1.1.284
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Rose ZB and Racker E (1966). "Formaldehyde dehydrogenase". Methods Enzymol. 9: 357–360.
Liu L, Hausladen A, Zeng M, Que L, Heitman J, Stamler JS (2001). "A metabolic enzyme for S-nitrosothiol conserved from bacteria to humans". Nature. 410: 490–4. doi:10.1038/35068596. PMID 11260719.
Sanghani PC, Stone CL, Ray BD, Pindel EV, Hurley TD, Bosron WF (2000). "Kinetic mechanism of human glutathione-dependent formaldehyde dehydrogenase". Biochemistry. 39: 10720–9. doi:10.1021/bi9929711. PMID 10978156.
Probst C, Grotz M, Krettek C, Pape HC (2005). "Impact of hypothermia on the immunologic response after trauma and elective surgery". Surg. Technol. Int. 14: 41–50. PMID 16525953.
Stouthamer AH, Harms N (1995). "Isolation, sequencing, and mutagenesis of the gene encoding NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) from Paracoccus denitrificans, in which GD-FALDH is essential for methylotrophic growth". J. Bacteriol. 177: 247–51. PMID 7798140.
Barber RD, Rott MA, Donohue TJ (1996). "Characterization of a glutathione-dependent formaldehyde dehydrogenase from Rhodobacter sphaeroides". J. Bacteriol. 178: 1386–93. PMID 8631716.