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RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA.[1] Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction.[2][3] This method often uses a proprietary formulation of this reagent called Trizol.
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[edit] References
- ^ Peirson SN, Butler JN (2007). "RNA extraction from mammalian tissues". Methods Mol. Biol. 362: 315–27. PMID 17417019.
- ^ Chomczynski P, Sacchi N (2006). "The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on". Nat Protoc 1 (2): 581–5. doi:10.1038/nprot.2006.83. PMID 17406285.
- ^ Bird IM (2005). "Extraction of RNA from cells and tissue". Methods Mol. Med. 108: 139–48. PMID 16028681.
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