Nucleic acid methods
From Wikipedia, the free encyclopedia
Nucleic acid methods are the techniques used to study Nucleic acids (DNA and RNA).
Purification
Quantification
- Abundance in weight: spectroscopic quantification
- Absolute abundance in number: RTPCR
- high-throughput relative abundance DNA microarray
- high-throughput absolute abundance SAGE
- Size: Gel electrophoresis
Synthesis
- De novo: Oligonucleotide synthesis
- Amplification: PCR
Other
- DNA sequencing
- Bisulfite sequencing
- Expression cloning
- Southern blot
- northern blot
- Sucrose gradient centrifugation
- Radioactivity in biological research
- Lab-on-a-chip
- Nuclear run-on assay
- Fluorescent in situ hybridization
- several Bioinformatics methods, such as RNA structure prediction
For protein methods, see Protein methods.
- Protocols and other How-to information are not present in Wikipedia (see WP:NOT#HOWTO), for these one could consult Open wetware website
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