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| Henry II and I of Leon |
| Children include |
| John I |
| Eleanor, Queen of Navarre |
| John I |
| Children include |
| Henry III of Castile and II of Leon) |
| Ferdinand I of Aragon, Valencia and Sicily |
| Henry III and II of Leon |
| Children include |
| John II |
| Maria, Queen of Aragon, Valencia, Sicily and Naples |
| John II |
| Children include |
| Henry IV of Castile and III of Leon |
| Isabella I |
| Alfonso, Prince of Asturias |
| Henry IV and III of Leon |
| Children |
| Joan, Queen of Portugal |
| Isabella I with Ferdinand V |
| Children |
| Isabella, Queen of Portugal |
| Juan, Prince of Asturias |
| Joanna I |
| Maria, Queen of Portugal |
| Catherine, Queen of England |
| Grandchildren include |
| Miguel da Paz, Prince of Portugal and Spain |
| Joanna with Philip I |
| Children |
| Eleanor, Queen of Portugal and France |
| Charles V, Holy Roman Emperor |
| Isabella, Queen of Denmark and Norway |
| Ferdinand I, Holy Roman Emperor |
| Mary, Queen of Hungary and Bohemia |
| Catherine, Queen of Portugal |