Glycoprotein 6-alpha-L-fucosyltransferase

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In enzymology, a glycoprotein 6-alpha-L-fucosyltransferase (EC 2.4.1.68) is an enzyme that catalyzes the chemical reaction

GDP-L-fucose + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D- glucosaminyl}asparagine $\rightleftharpoons$ GDP + N₄-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N- acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D- mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L- fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl}asparagine

The 5 substrates of this enzyme are GDP-L-fucose, [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-]], [[mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-]], and [[glucosaminyl}asparagine]], whereas its 5 products are GDP, [[N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-]], [[acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-]], [[mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-[alpha-L-]], and [[fucosyl-(1->6)]-N-acetyl-beta-D-glucosaminyl}asparagine]].

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-a cetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-man nosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glu cosaminyl}asparagine) 6-alpha-L-fucosyltransferase. Other names in common use include GDP-fucose-glycoprotein fucosyltransferase, GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1->6fucosyltransferase, GDP-L-fucose-glycoprotein fucosyltransferase, glycoprotein fucosyltransferase, guanosine diphosphofucose-glycoprotein fucosyltransferase, GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked, N-acetylglucosamine of, 4-N-{N-acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->3)-[N-, acetyl-beta-D-glucosaminyl-(1->2)-alpha-D-mannosyl-(1->6)]-beta-D-, mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-, glucosaminyl}asparagine) 6-alpha-L-fucosyltransferase, and FucT. This enzyme participates in 3 metabolic pathways: n-glycan biosynthesis, keratan sulfate biosynthesis, and glycan structures - biosynthesis 1.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DE0, 2HHC, and 2HLH.

[edit] References

IUBMB entry for 2.4.1.68
BRENDA references for 2.4.1.68 (Recommended.)
PubMed references for 2.4.1.68
PubMed Central references for 2.4.1.68
Google Scholar references for 2.4.1.68
Longmore GD, Schachter H (1982). "Product-identification and substrate-specificity studies of the GDP-L-fucose:2-acetamido-2-deoxy-beta-D-glucoside (FUC goes to Asn-linked GlcNAc) 6-alpha-L-fucosyltransferase in a Golgi-rich fraction from porcine liver". Carbohydr. Res. 100: 365–92. doi:10.1016/S0008-6215(00)81049-6. PMID 7083256.
Voynow JA, Scanlin TF, Glick MC (1988). "A quantitative method for GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha 1--6fucosyltransferase activity with lectin affinity chromatography". Anal. Biochem. 168: 367–73. doi:10.1016/0003-2697(88)90331-4. PMID 3364733.
Teshima T, Fujii S, Shiba T, Taniguchi N (1996). "Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-->6fucosyltransferase". J. Biol. Chem. 271: 27810–7. doi:10.1074/jbc.271.44.27810. PMID 8910378.