Talk:Denaturation (biochemistry)

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I find the word "denaturating" commonly used, but it would appear to be incorrect... Would any biochemistry experts out there like to comment on a possible distinction between denaturing and denaturating? Is this error as widespread as it seems, or is a "denaturating buffer" different in some way from a "denaturing buffer?"

[edit] From Talk:Denatured

Is gelatin denatured collagen? Both have very high glycine content. -phma

  • since it is capable of a gel to sol transition, I would have to say no. This is a blurb about gelatin from http://www.gelatine.org :

Gelatine is a pure protein obtained from animal raw materials containing collagen. The natural and healthy food has excellent gelling strength.

Dwmyers 16:16 Feb 12, 2003 (UTC)

Another example that could be added, if it isn't too grisly: if my information is correct, denaturation is why you wash blood stains in cold water. Using hot water on blood denatures it, causing protein strands to twist around fibers in the fabric, making them almost impossible to remove. Using cold water to clean blood out of cloth works pretty well.

I heard that you can prepare fish like tuna and salmon by marinating them in lemon juice over night as opposed to cooking with heat. Anyone know more information about this? I assume it's an example of denaturation.

same as the ceviche example


[edit] H-bonding in secondary structure

I believe that it should be noted that the disruption of secondary structure is due to the breaking of hydrogen bonds between the amino acid residues. This is important because it is the localized hydrogen bonding that lets amino acids form the secondary structures that they do. —Preceding unsigned comment added by 130.126.68.140 (talk) 22:02, 18 September 2007 (UTC)


[edit] Spamming Wikipedia

Biologicalworld.com has spammed wikipedia like no tomorrow. He is a site of only a few pages and a LOT of adsense. Not much information is given except for "protocols" which are not referenced, and cannot be trusted from a site of that quality.

check: Links from Wikipedia

Please help me with this someone or get a bot to delete the spam.

The following have been cleaned up:

  • en.wikipedia.org/wiki/Plasmid
  • en.wikipedia.org/wiki/Gel_electrophoresis
  • en.wikipedia.org/wiki/Green_fluorescent_protein
  • en.wikipedia.org/wiki/Homology_(biology)
  • en.wikipedia.org/wiki/HeLa
  • en.wikipedia.org/wiki/Protease
  • en.wikipedia.org/wiki/Restriction_enzyme
  • en.wikipedia.org/wiki/Petri_dish
  • en.wikipedia.org/wiki/Structural_domain
  • en.wikipedia.org/wiki/Trypsin
  • en.wikipedia.org/wiki/Oligonucleotide
  • en.wikipedia.org/wiki/Transmission_electron_microscope
  • en.wikipedia.org/wiki/Agar_plate
  • en.wikipedia.org/wiki/Calcium_phosphate
  • en.wikipedia.org/wiki/Disulfide_bond
  • en.wikipedia.org/wiki/Denaturation_(biochemistry)
  • en.wikipedia.org/wiki/DNA_ligase
  • en.wikipedia.org/wiki/Wild_type
  • en.wikipedia.org/wiki/Tissue_culture
  • en.wikipedia.org/wiki/Transmission_electron_microscopy
  • en.wikipedia.org/wiki/Reporter_gene
  • en.wikipedia.org/wiki/Northern_blot
  • en.wikipedia.org/wiki/Protein_engineering
  • en.wikipedia.org/wiki/Sticky_end/blunt_end
  • en.wikipedia.org/wiki/Taq_polymerase
  • en.wikipedia.org/wiki/Protein_domain
  • en.wikipedia.org/wiki/Coomassie
  • en.wikipedia.org/wiki/Native_state
  • en.wikipedia.org/wiki/Chinese_Hamster_Ovary_cell
  • en.wikipedia.org/wiki/Peptidase
  • en.wikipedia.org/wiki/Visking_tubing
  • en.wikipedia.org/wiki/Streptavidin
  • en.wikipedia.org/wiki/Microtiter_plate
  • en.wikipedia.org/wiki/Subcloning
  • en.wikipedia.org/wiki/Ion_exchange_chromatography
  • en.wikipedia.org/wiki/Thermal_cycler
  • en.wikipedia.org/wiki/Bovine_serum_albumin
  • en.wikipedia.org/wiki/Phosphate_buffered_saline
  • en.wikipedia.org/wiki/Glutathione_S-transferase
  • en.wikipedia.org/wiki/HEPES
  • en.wikipedia.org/wiki/Ortholog
  • en.wikipedia.org/wiki/Proteases
  • en.wikipedia.org/wiki/Salting_out
  • en.wikipedia.org/wiki/Fetal_bovine_serum
  • en.wikipedia.org/wiki/Proteolytic_enzyme
  • en.wikipedia.org/wiki/DNA_end
  • en.wikipedia.org/wiki/Supernatant
  • en.wikipedia.org/wiki/ABTS
  • en.wikipedia.org/wiki/Conserved_sequence
  • en.wikipedia.org/wiki/Peptide_mass_fingerprinting
  • en.wikipedia.org/wiki/Dithiothreitol
  • en.wikipedia.org/wiki/Uranyl_acetate

and many more Sciencetalks (talk) —Preceding comment was added at 03:05, 4 January 2008 (UTC)

If you find a URL that is added as spam to articles you can take it to m:Talk:Spam blacklist. ----Seans Potato Business 12:58, 28 January 2008 (UTC)