Azobenzene reductase

From Wikipedia, the free encyclopedia

In enzymology, an azobenzene reductase (EC 1.7.1.6) is an enzyme that catalyzes the chemical reaction

N,N-dimethyl-1,4-phenylenediamine + aniline + NADP⁺ $\rightleftharpoons$ 4-(dimethylamino)azobenzene + NADPH + H⁺

The 3 substrates of this enzyme are N,N-dimethyl-1,4-phenylenediamine, aniline, and NADP⁺, whereas its 3 products are 4-(dimethylamino)azobenzene, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on other nitrogenous compounds as donors with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is N,N-dimethyl-1,4-phenylenediamine, aniline:NADP+ oxidoreductase. Other names in common use include new coccine (NC)-reductase, NC-reductase, azo-dye reductase, orange II azoreductase, NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase, orange I azoreductase, azo reductase, azoreductase, nicotinamide adenine dinucleotide (phosphate) azoreductase, NADPH2-dependent azoreductase, dimethylaminobenzene reductase, p-dimethylaminoazobenzene azoreductase, dibromopropylaminophenylazobenzoic azoreductase, N,N-dimethyl-4-phenylazoaniline azoreductase, p-aminoazobenzene reductase, methyl red azoreductase, and NADPH2:4-(dimethylamino)azobenzene oxidoreductase.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 1NNI, 1V4B, and 2D5I.

[edit] References

IUBMB entry for 1.7.1.6
BRENDA references for 1.7.1.6 (Recommended.)
PubMed references for 1.7.1.6
PubMed Central references for 1.7.1.6
Google Scholar references for 1.7.1.6
Mueller GC and Miller JA (1949). "The reductive cleavage of 4-dimethylaminoazobenzene by rat liver: the intracellular distribution of the enzyme system and its requirements for triphosphopyridine nucleotide". J. Biol. Chem. 180: 1125–1136.
Suzuki Y, Yoda T, Ruhul A, Sugiura W (2001). "Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil". J. Biol. Chem. 276: 9059–65. doi:10.1074/jbc.M008083200. PMID 11134015.