3beta-hydroxy-5beta-steroid dehydrogenase

From Wikipedia, the free encyclopedia

In enzymology, a 3beta-hydroxy-5beta-steroid dehydrogenase (EC 1.1.1.277) is an enzyme that catalyzes the chemical reaction

3beta-hydroxy-5beta-pregnane-20-one + NADP⁺ $\rightleftharpoons$ 5beta-pregnan-3,20-dione + NADPH + H⁺

Thus, the two substrates of this enzyme are 3beta-hydroxy-5beta-pregnane-20-one and NADP⁺, whereas its 3 products are 5beta-pregnan-3,20-dione, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3beta-hydroxy-5beta-steroid:NADP+ 3-oxidoreductase. Other names in common use include 3beta-hydroxysteroid 5beta-oxidoreductase, and 3beta-hydroxysteroid 5beta-progesterone oxidoreductase.

[edit] References

IUBMB entry for 1.1.1.277
BRENDA references for 1.1.1.277 (Recommended.)
PubMed references for 1.1.1.277
PubMed Central references for 1.1.1.277
Google Scholar references for 1.1.1.277
Stuhlemmer U and Kreis W (1996). "Cardenolide formation and activity of pregnane-modifying enzymes in cell suspension cultures, shoot cultures and leaves of Digitalis lanata". Plant Physiol. 34: 85–91.
Seitz HU and Gaertner DE (1994). "Enzymes in cardenolide-accumulating shoot cultures of Digitalis purpurea". Plant Cell 38: 337–344.
Lindemann P and Luckner M (1997). "Biosynthesis of pregnane derivatives in somatic embryos of Digitalis lanata". Phytochemistry 46: 507–513. doi:10.1016/S0031-9422(97)00315-4.