3-keto-steroid reductase

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In enzymology, a 3-keto-steroid reductase (EC 1.1.1.270) is an enzyme that catalyzes the chemical reaction

4alpha-methyl-5alpha-cholest-7-en-3beta-ol + NADP⁺ $\rightleftharpoons$ 4alpha-methyl-5alpha-cholest-7-en-3-one + NADPH + H⁺

Thus, the two substrates of this enzyme are 4alpha-methyl-5alpha-cholest-7-en-3beta-ol and NADP⁺, whereas its 3 products are 4alpha-methyl-5alpha-cholest-7-en-3-one, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 3beta-hydroxy-steroid:NADP+ 3-oxidoreductase. This enzyme is also called 3-KSR. This enzyme participates in biosynthesis of steroids.

[edit] References

IUBMB entry for 1.1.1.270
BRENDA references for 1.1.1.270 (Recommended.)
PubMed references for 1.1.1.270
PubMed Central references for 1.1.1.270
Google Scholar references for 1.1.1.270
Billheimer JT, Alcorn M, Gaylor JL (1981). "Solubilization and partial purification of a microsomal 3-ketosteroid reductase of cholesterol biosynthesis". Arch. Biochem. Biophys. 211: 430–8. doi:10.1016/0003-9861(81)90474-4. PMID 6946726.
Swindell AC, Gaylor JL (1968). "Investigation of the component reactions of oxidative sterol demethylation. Formation and metabolism of 3-ketosteroid intermediates". J. Biol. Chem. 243: 5546–55. PMID 4387005.