1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming)

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In enzymology, a 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) (EC 1.1.1.292) is an enzyme that catalyzes the chemical reaction

1,5-anhydro-D-mannitol + NADP⁺ $\rightleftharpoons$ 1,5-anhydro-D-fructose + NADPH + H⁺

Thus, the two substrates of this enzyme are 1,5-anhydro-D-mannitol and NADP⁺, whereas its 3 products are 1,5-anhydro-D-fructose, NADPH, and H⁺.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is 1,5-anhydro-D-mannitol:NADP+ oxidoreductase. Other names in common use include 1,5-anhydro-D-fructose reductase (ambiguous), and AFR.

[edit] References

IUBMB entry for 1.1.1.292
BRENDA references for 1.1.1.292 (Recommended.)
PubMed references for 1.1.1.292
PubMed Central references for 1.1.1.292
Google Scholar references for 1.1.1.292
Kuhn A, Yu S, Giffhorn F (2006). "Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis". Appl. Environ. Microbiol. 72: 1248–57. PMID 16461673.
Dambe TR, Kuhn AM, Brossette T, Giffhorn F, Scheidig AJ (2006). "Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 A resolution: construction of an NADH-accepting mutant and its application in rare sugar synthesis". Biochemistry. 45: 10030–42. PMID 16906761.