UPF1

From Wikipedia, the free encyclopedia

UPF1 regulator of nonsense transcripts homolog (yeast)

PDB rendering based on 2gjk.

Available structures: 2gjk, 2gk6, 2gk7, 2iyk

Identifiers

Symbol(s)

UPF1; FLJ43809; FLJ46894; HUPF1; KIAA0221; NORF1; RENT1; pNORF1

External IDs

OMIM: 601430 MGI: 107995 HomoloGene: 2185

Gene ontology
Molecular Function:	• nucleotide binding • chromatin binding • RNA binding • ATP-dependent RNA helicase activity • helicase activity • protein binding • ATP binding • zinc ion binding • hydrolase activity • metal ion binding
Cellular Component:	• chromatin • cytoplasm
Biological Process:	• mRNA catabolic process, nonsense-mediated decay • DNA replication • DNA repair • RNA processing • mRNA export from nucleus • regulation of translational termination • cell cycle

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_002911 (mRNA)
NP_002902 (protein)

NM_030680 (mRNA)
NP_109605 (protein)

Location

Chr 19: 18.8 - 18.84 Mb

Chr 8: 73.26 - 73.28 Mb

Pubmed search

[1]

[2]

UPF1 regulator of nonsense transcripts homolog (yeast), also known as UPF1, is a human gene.

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.^[1]

[edit] References

^ Entrez Gene: UPF1 UPF1 regulator of nonsense transcripts homolog (yeast).

[edit] Further reading

Azzalin CM, Lingner J (2006). "The double life of UPF1 in RNA and DNA stability pathways.". Cell Cycle 5 (14): 1496-8. PMID 16861888.
Horikoshi M, Sekimizu K, Natori S (1984). "Analysis of the stimulatory factor of RNA polymerase II in the initiation and elongation complex.". J. Biol. Chem. 259 (1): 608-11. PMID 6200476.
Perlick HA, Medghalchi SM, Spencer FA, et al. (1996). "Mammalian orthologues of a yeast regulator of nonsense transcript stability.". Proc. Natl. Acad. Sci. U.S.A. 93 (20): 10928-32. PMID 8855285.
Nagase T, Seki N, Ishikawa K, et al. (1997). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.". DNA Res. 3 (5): 321-9, 341-54. PMID 9039502.
Applequist SE, Selg M, Raman C, Jäck HM (1997). "Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein.". Nucleic Acids Res. 25 (4): 814-21. PMID 9064659.
Czaplinski K, Ruiz-Echevarria MJ, Paushkin SV, et al. (1998). "The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs.". Genes Dev. 12 (11): 1665-77. PMID 9620853.
Page MF, Carr B, Anders KR, et al. (1999). "SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast.". Mol. Cell. Biol. 19 (9): 5943-51. PMID 10454541.
Bhattacharya A, Czaplinski K, Trifillis P, et al. (2000). "Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay.". RNA 6 (9): 1226-35. PMID 10999600.
Mendell JT, Medghalchi SM, Lake RG, et al. (2000). "Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes.". Mol. Cell. Biol. 20 (23): 8944-57. PMID 11073994.
Serin G, Gersappe A, Black JD, et al. (2001). "Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4).". Mol. Cell. Biol. 21 (1): 209-23. doi:10.1128/MCB.21.1.209-223.2001. PMID 11113196.
Lykke-Andersen J, Shu MD, Steitz JA (2001). "Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon.". Cell 103 (7): 1121-31. PMID 11163187.
Denning G, Jamieson L, Maquat LE, et al. (2001). "Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein.". J. Biol. Chem. 276 (25): 22709-14. doi:10.1074/jbc.C100144200. PMID 11331269.
Yamashita A, Ohnishi T, Kashima I, et al. (2001). "Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay.". Genes Dev. 15 (17): 2215-28. doi:10.1101/gad.913001. PMID 11544179.
Lykke-Andersen J, Shu MD, Steitz JA (2001). "Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.". Science 293 (5536): 1836-9. doi:10.1126/science.1062786. PMID 11546874.
Carastro LM, Tan CK, Selg M, et al. (2002). "Identification of delta helicase as the bovine homolog of HUPF1: demonstration of an interaction with the third subunit of DNA polymerase delta.". Nucleic Acids Res. 30 (10): 2232-43. PMID 12000843.
Mendell JT, ap Rhys CM, Dietz HC (2002). "Separable roles for rent1/hUpf1 in altered splicing and decay of nonsense transcripts.". Science 298 (5592): 419-22. doi:10.1126/science.1074428. PMID 12228722.
Lykke-Andersen J (2003). "Identification of a human decapping complex associated with hUpf proteins in nonsense-mediated decay.". Mol. Cell. Biol. 22 (23): 8114-21. PMID 12417715.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Chiu SY, Serin G, Ohara O, Maquat LE (2003). "Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1.". RNA 9 (1): 77-87. PMID 12554878.
Schell T, Köcher T, Wilm M, et al. (2003). "Complexes between the nonsense-mediated mRNA decay pathway factor human upf1 (up-frameshift protein 1) and essential nonsense-mediated mRNA decay factors in HeLa cells.". Biochem. J. 373 (Pt 3): 775-83. doi:10.1042/BJ20021920. PMID 12723973.