Ubiquitin-protein ligase

From Wikipedia, the free encyclopedia

In enzymology, an ubiquitin-protein ligase (EC 6.3.2.19) is an enzyme that catalyzes the chemical reaction

ATP + ubiquitin + protein lysine $\rightleftharpoons$ AMP + diphosphate + protein N-ubiquityllysine

The 3 substrates of this enzyme are ATP, ubiquitin, and protein lysine, whereas its 3 products are AMP, diphosphate, and protein N-ubiquityllysine.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is ubiquitin:protein-lysine N-ligase (AMP-forming). This enzyme is also called ubiquitin-activating enzyme. This enzyme participates in 3 metabolic pathways: ubiquitin mediated proteolysis, parkinson's disease, and huntington's disease.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 75 structures have been solved for this class of enzymes, with PDB accession codes 1A3S, 1AYZ, 1C4Z, 1FBV, 1FXT, 1FZY, 1I7K, 1IYF, 1J7D, 1JAS, 1JAT, 1JBB, 1KPS, 1PZV, 1Q34, 1QCQ, 1TTE, 1U9A, 1U9B, 1UMH, 1UMI, 1UR6, 1W4U, 1WD2, 1WZV, 1WZW, 1X23, 1XR9, 1Y6L, 1Y8X, 1YH6, 1YLA, 1YRV, 1Z2U, 1Z3D, 1Z5S, 1Z6U, 1ZDN, 1ZUO, 2A4D, 2A7L, 2AAK, 2AWF, 2AYV, 2BEP, 2BF8, 2C4O, 2C4P, 2CLW, 2CYX, 2E2C, 2EKE, 2ESK, 2ESO, 2ESP, 2ESQ, 2F4W, 2FO3, 2FUH, 2GJD, 2GMI, 2GRN, 2GRO, 2GRP, 2GRQ, 2GRR, 2H2Y, 2O25, 2OB4, 2OGB, 2ONU, 2UCZ, 2UYZ, 2Z6O, and 2Z6P.

[edit] References

IUBMB entry for 6.3.2.19
BRENDA references for 6.3.2.19 (Recommended.)
PubMed references for 6.3.2.19
PubMed Central references for 6.3.2.19
Google Scholar references for 6.3.2.19
Ciechanover A, Elias S, Heller H, Hershko A (1982). ""Covalent affinity" purification of ubiquitin-activating enzyme". J. Biol. Chem. 257: 2537–42. PMID 6277904.
Haas AL, Rose IA (1982). "The mechanism of ubiquitin activating enzyme. A kinetic and equilibrium analysis". J. Biol. Chem. 257: 10329–37. PMID 6286650.
Haas AL, Warms JV, Hershko A, Rose IA (1982). "Ubiquitin-activating enzyme. Mechanism and role in protein-ubiquitin conjugation". J. Biol. Chem. 257: 2543–8. PMID 6277905.
Hershko A, Heller H, Eytan E, Reiss Y (1986). "The protein substrate binding site of the ubiquitin-protein ligase system". J. Biol. Chem. 261: 11992–9. PMID 3017957.