UBE2B

From Wikipedia, the free encyclopedia

Ubiquitin-conjugating enzyme E2B (RAD6 homolog)

PDB rendering based on 1jas.

Available structures: 1jas

Identifiers

Symbol(s)

UBE2B; UBC2; E2-17kDa; HHR6B; HR6B; RAD6B

External IDs

OMIM: 179095 MGI: 102944 HomoloGene: 37761

Gene ontology
Molecular Function:	• ubiquitin-protein ligase activity • protein binding • ligase activity
Cellular Component:	• chromatin • nucleus • membrane
Biological Process:	• postreplication repair • ubiquitin-dependent protein catabolic process • ubiquitin cycle • sperm axoneme assembly

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_003337 (mRNA)
NP_003328 (protein)

NM_009458 (mRNA)
NP_033484 (protein)

Location

Chr 5: 133.73 - 133.76 Mb

Chr 11: 51.83 - 51.84 Mb

Pubmed search

[1]

[2]

Ubiquitin-conjugating enzyme E2B (RAD6 homolog), also known as UBE2B, is a human gene.^[1]

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.^[1]

[edit] References

^ ^a ^b Entrez Gene: UBE2B ubiquitin-conjugating enzyme E2B (RAD6 homolog).

[edit] Further reading

Koken MH, Smit EM, Jaspers-Dekker I, et al. (1992). "Localization of two human homologs, HHR6A and HHR6B, of the yeast DNA repair gene RAD6 to chromosomes Xq24-q25 and 5q23-q31.". Genomics 12 (3): 447–53. PMID 1559696.
Koken MH, Reynolds P, Jaspers-Dekker I, et al. (1991). "Structural and functional conservation of two human homologs of the yeast DNA repair gene RAD6.". Proc. Natl. Acad. Sci. U.S.A. 88 (20): 8865–9. PMID 1717990.
Woffendin C, Chen ZY, Staskus K, et al. (1991). "Mammalian mRNAs encoding protein closely related to ubiquitin-conjugating enzyme encoded by yeast DNA repair gene RAD6.". Biochim. Biophys. Acta 1090 (1): 81–5. PMID 1883845.
Schneider R, Eckerskorn C, Lottspeich F, Schweiger M (1990). "The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6.". EMBO J. 9 (5): 1431–5. PMID 2158443.
Koken MH, Hoogerbrugge JW, Jasper-Dekker I, et al. (1996). "Expression of the ubiquitin-conjugating DNA repair enzymes HHR6A and B suggests a role in spermatogenesis and chromatin modification.". Dev. Biol. 173 (1): 119–32. PMID 8575614.
Tateishi S, Sakuraba Y, Masuyama S, et al. (2000). "Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.". Proc. Natl. Acad. Sci. U.S.A. 97 (14): 7927–32. PMID 10884424.
Xin H, Lin W, Sumanasekera W, et al. (2000). "The human RAD18 gene product interacts with HHR6A and HHR6B.". Nucleic Acids Res. 28 (14): 2847–54. PMID 10908344.
Miura T, Klaus W, Ross A, et al. (2003). "The NMR structure of the class I human ubiquitin-conjugating enzyme 2b.". J. Biomol. NMR 22 (1): 89–92. PMID 11885984.
Shekhar MP, Lyakhovich A, Visscher DW, et al. (2002). "Rad6 overexpression induces multinucleation, centrosome amplification, abnormal mitosis, aneuploidy, and transformation.". Cancer Res. 62 (7): 2115–24. PMID 11929833.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Kwon YT, Xia Z, An JY, et al. (2003). "Female lethality and apoptosis of spermatocytes in mice lacking the UBR2 ubiquitin ligase of the N-end rule pathway.". Mol. Cell. Biol. 23 (22): 8255–71. PMID 14585983.
Lyakhovich A, Shekhar MP (2004). "RAD6B overexpression confers chemoresistance: RAD6 expression during cell cycle and its redistribution to chromatin during DNA damage-induced response.". Oncogene 23 (17): 3097–106. doi:10.1038/sj.onc.1207449. PMID 14981545.
Watanabe K, Tateishi S, Kawasuji M, et al. (2005). "Rad18 guides poleta to replication stalling sites through physical interaction and PCNA monoubiquitination.". EMBO J. 23 (19): 3886–96. doi:10.1038/sj.emboj.7600383. PMID 15359278.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome.". Cell 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. PMID 16169070.
Shekhar MP, Tait L, Gerard B (2007). "Essential role of T-cell factor/beta-catenin in regulation of Rad6B: a potential mechanism for Rad6B overexpression in breast cancer cells.". Mol. Cancer Res. 4 (10): 729–45. doi:10.1158/1541-7786.MCR-06-0136. PMID 17050667.