U2AF2

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U2 small nuclear RNA auxiliary factor 2
PDB rendering based on 1jmt.
Available structures: 1jmt, 1o0p, 1opi, 1u2f, 2hzc, 2u2f
Identifiers
Symbol(s) U2AF2; U2AF65
External IDs OMIM: 191318 HomoloGene: 5267
RNA expression pattern

More reference expression data
Orthologs
Human Mouse
Entrez 11338 n/a
Ensembl ENSG00000063244 n/a
Uniprot P26368 n/a
Refseq NM_001012478 (mRNA)
NP_001012496 (protein)		 n/a (mRNA)
n/a (protein)
Location Chr 19: 60.86 - 60.88 Mb n/a
Pubmed search [1] n/a

U2 small nuclear RNA auxiliary factor 2, also known as U2AF2, is a human gene.

U2 auxiliary factor (U2AF), comprised of a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the U2AF large subunit which contains a sequence-specific RNA-binding region with 3 RNA recognition motifs and an Arg/Ser-rich domain necessary for splicing. The large subunit binds to the polypyrimidine tract of introns early during spliceosome assembly. Multiple transcript variants have been detected for this gene, but the full-length natures of only two have been determined to date.[1]

[edit] References

  1. ^ Entrez Gene: U2AF2 U2 small nuclear RNA auxiliary factor 2.
  • Zhang M, Zamore PD, Carmo-Fonseca M, et al. (1992). "Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit.". Proc. Natl. Acad. Sci. U.S.A. 89 (18): 8769–73. PMID 1388271. 
  • Zamore PD, Patton JG, Green MR (1992). "Cloning and domain structure of the mammalian splicing factor U2AF.". Nature 355 (6361): 609–14. doi:10.1038/355609a0. PMID 1538748. 
  • Zamore PD, Green MR (1991). "Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.". EMBO J. 10 (1): 207–14. PMID 1824937. 
  • Zuo P, Maniatis T (1996). "The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing.". Genes Dev. 10 (11): 1356–68. PMID 8647433. 
  • Zhang WJ, Wu JY (1996). "Functional properties of p54, a novel SR protein active in constitutive and alternative splicing.". Mol. Cell. Biol. 16 (10): 5400–8. PMID 8816452. 
  • Hong W, Bennett M, Xiao Y, et al. (1997). "Association of U2 snRNP with the spliceosomal complex E.". Nucleic Acids Res. 25 (2): 354–61. PMID 9016565. 
  • Abovich N, Rosbash M (1997). "Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals.". Cell 89 (3): 403–12. PMID 9150140. 
  • Tronchère H, Wang J, Fu XD (1997). "A protein related to splicing factor U2AF35 that interacts with U2AF65 and SR proteins in splicing of pre-mRNA.". Nature 388 (6640): 397–400. doi:10.1038/41137. PMID 9237760. 
  • Fleckner J, Zhang M, Valcárcel J, Green MR (1997). "U2AF65 recruits a novel human DEAD box protein required for the U2 snRNP-branchpoint interaction.". Genes Dev. 11 (14): 1864–72. PMID 9242493. 
  • Zhang WJ, Wu JY (1998). "Sip1, a novel RS domain-containing protein essential for pre-mRNA splicing.". Mol. Cell. Biol. 18 (2): 676–84. PMID 9447963. 
  • Wang HY, Lin W, Dyck JA, et al. (1998). "SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells.". J. Cell Biol. 140 (4): 737–50. PMID 9472028. 
  • Berglund JA, Abovich N, Rosbash M (1998). "A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition.". Genes Dev. 12 (6): 858–67. PMID 9512519. 
  • Rudner DZ, Kanaar R, Breger KS, Rio DC (1998). "Interaction between subunits of heterodimeric splicing factor U2AF is essential in vivo.". Mol. Cell. Biol. 18 (4): 1765–73. PMID 9528748. 
  • Lallena MJ, Martínez C, Valcárcel J, Correas I (1998). "Functional association of nuclear protein 4.1 with pre-mRNA splicing factors.". J. Cell. Sci. 111 ( Pt 14): 1963–71. PMID 9645944. 
  • Gozani O, Potashkin J, Reed R (1998). "A potential role for U2AF-SAP 155 interactions in recruiting U2 snRNP to the branch site.". Mol. Cell. Biol. 18 (8): 4752–60. PMID 9671485. 
  • Neubauer G, King A, Rappsilber J, et al. (1998). "Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex.". Nat. Genet. 20 (1): 46–50. doi:10.1038/1700. PMID 9731529. 
  • Davies RC, Calvio C, Bratt E, et al. (1998). "WT1 interacts with the splicing factor U2AF65 in an isoform-dependent manner and can be incorporated into spliceosomes.". Genes Dev. 12 (20): 3217–25. PMID 9784496. 
  • Ito T, Muto Y, Green MR, Yokoyama S (1999). "Solution structures of the first and second RNA-binding domains of human U2 small nuclear ribonucleoprotein particle auxiliary factor (U2AF(65)).". EMBO J. 18 (16): 4523–34. doi:10.1093/emboj/18.16.4523. PMID 10449418. 
  • Wang X, Bruderer S, Rafi Z, et al. (1999). "Phosphorylation of splicing factor SF1 on Ser20 by cGMP-dependent protein kinase regulates spliceosome assembly.". EMBO J. 18 (16): 4549–59. doi:10.1093/emboj/18.16.4549. PMID 10449420. 
  • Ladomery MR, Slight J, Mc Ghee S, Hastie ND (2000). "Presence of WT1, the Wilm's tumor suppressor gene product, in nuclear poly(A)(+) ribonucleoprotein.". J. Biol. Chem. 274 (51): 36520–6. PMID 10593950. 
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