Talk:Two-dimensional gel electrophoresis
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"Since the SDS molecules are negatively charged, the result of this is that all of the proteins will have approximately the same mass-to-charge ratio as each other. Next, an electric potential is again applied, but at a 90 degree angle from the first field. The proteins will be attracted to the more negative side of the gel proportionally to their mass-to-charge ratio."
If it's negatively charged, wouldn't it be attracted to the positif side instead of the negative one ?
That is exactly my concern as well.
SDS does impart a negative charge to the particle, but is then subsequently attracted to the POSITIVE pole when a charge is applied to the gel.
Also, strictly shouldn't it be size/charge not mass/charge?
The technique was introduced by Klose, 1975; O'Farrell, 1975; Scheele, 1975. Earlier publications did not include a detergent in the second dimension.
[edit] What about 2D Blue native page?
This article assumes that the only 1st dimension PAGE technique available is isoelectrofocussing; in my knowledge, there are other valid 1st dimension PAGEs which are commonly used (see: blue native PAGE, for instance). Another technique I read about in various papers (viral protein separation) used a 2D PAGE where the first dimension used a cathionic detergent. This article would greatly benefit from a broader approach.
