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[edit] Summary

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Description

Overview of two-hybrid assay, checking for interactions between two proteins, called here Bait and Prey.
A. Gal4 transcription factor gene produces two domain protein (BD and AD) which is essential for transcription of the reporter gene (LacZ).
B,C. Two fusion proteins are prepared: Gal4BD+Bait and Gal4AD+Prey. None of them is usually sufficient to initiate the transcription (of the reporter gene) alone.
D. When both fusion proteins are produced and Bait part of the first interact with Prey part of the second, transcription of the reporter gene occurs.
Source

own work
Date

October 2007
Author

Anna
Permission
(Reusing this image)

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[edit] Licensing

I, the copyright holder of this work, hereby publish it under the following licenses:
GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation license, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled "GNU Free Documentation license".

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File history

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Date/TimeDimensionsUserComment
current18:15, 9 October 2007709×886 (86 KB)Anna K. (Converted to plain SVG with some objects converted to paths.)
17:34, 9 October 2007709×886 (44 KB)Anna K. ({{Inkscape}} {{Information |Description=Two hybrid assay |Source=own work |Date=October 2007 |Author=Anna |other_versions= }} {{Translation possible}} Category:SVG Category:Diagrams in English)
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