tRNA guanosine-2'-O-methyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a tRNA guanosine-2'-O-methyltransferase (EC 2.1.1.34) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing 2'-O-methylguanosine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing 2'-O-methylguanosine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA guanosine-2'-O-methyltransferase. Other names in common use include transfer ribonucleate guanosine 2'-methyltransferase, tRNA guanosine 2'-methyltransferase, tRNA (guanosine 2')-methyltransferase, tRNA (Gm18) 2'-O-methyltransferase, tRNA (Gm18) methyltransferase, tRNA (guanosine-2'-O-)-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanosine-2'-O-)-methyltransferase.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1V2X and 1ZJR.

[edit] References

• IUBMB entry for 2.1.1.34
• BRENDA references for 2.1.1.34 (Recommended.)
• PubMed references for 2.1.1.34
• PubMed Central references for 2.1.1.34
• Google Scholar references for 2.1.1.34
• Gefter ML (1969). "The in vitro synthesis of 2'-omethylguanosine and 2-methylthio 6N (gamma,gamma, dimethylallyl) adenosine in transfer RNA of Escherichia coli". Biochem. Biophys. Res. Commun. 36: 435–41. PMID 4898378. 
• Kumagai I, Watanabe K, Oshima T (1980). "Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27". Proc. Natl. Acad. Sci. U. S. A. 77: 1922–6. PMID 6990416. 
• Kumagai I, Watanabe K (1998). "Substrate recognition of tRNA (Guanosine-2'-)-methyltransferase from Thermus thermophilus HB27". J. Biol. Chem. 273: 25721–7. PMID 9748240. 

[edit] External links

The CAS registry number for this enzyme class is 37257-01-5.

• IUBMB entry for 2.1.1.34

• KEGG entry for 2.1.1.34

• BRENDA entry for 2.1.1.34

• NiceZyme view of 2.1.1.34

• EC2PDB: PDB structures for 2.1.1.34

• PRIAM entry for 2.1.1.34

• PUMA2 entry for 2.1.1.34

• IntEnz: Integrated Enzyme entry for 2.1.1.34

• MetaCyc entry for 2.1.1.34

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0009020: tRNA (guanosine-2'-O-)-methyltransferase

• AMIGO entry for GO code 0009020: tRNA (guanosine-2'-O-)-methyltransferase