tRNA (guanine-N7-)-methyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a tRNA (guanine-N7-)-methyltransferase (EC 2.1.1.33) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA $\rightleftharpoons$ S-adenosyl-L-homocysteine + tRNA containing N₇-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N7-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 7-methyltransferase, 7-methylguanine transfer ribonucleate methylase, tRNA guanine 7-methyltransferase, N7-methylguanine methylase, and S-adenosyl-L-methionine:tRNA (guanine-7-N-)-methyltransferase.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1YZH and 2FCA.

[edit] References

IUBMB entry for 2.1.1.33
BRENDA references for 2.1.1.33 (Recommended.)
PubMed references for 2.1.1.33
PubMed Central references for 2.1.1.33
Google Scholar references for 2.1.1.33
Hurwitz J, Gold M, Anders M (1964). "The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes". J. Biol. Chem. 239: 3462–3473. PMID 14245404.
Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9: 207–215. PMID 69267419.
Aschhoff HJ, Elten H, Arnold HH, Mahal G, Kersten W, Kersten H (1976). "7-Methylguanine specific tRNA-methyltransferase from Escherichia coli". Nucleic. Acids. Res. 3: 3109–22. PMID 794833.
Salvatore F, Traboni C, Colonna A, Ciliberto G, Paoletta C, Cimino F (1980). in Cavallini D, Gaul GE, Zappia V: Natural Sulfur Compounds. New York: Plenum Press, p. 25.