tRNA (guanine-N2-)-methyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a tRNA (guanine-N2-)-methyltransferase (EC 2.1.1.32) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA $\rightleftharpoons$ S-adenosyl-L-homocysteine + tRNA containing N₂-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N2-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N2-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 2-methyltransferase, transfer ribonucleate guanine N2-methyltransferase, transfer RNA guanine 2-methyltransferase, guanine-N2-methylase, and S-adenosyl-L-methionine:tRNA (guanine-2-N-)-methyltransferase.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2DUL, 2EJT, and 2EJU.

[edit] References

IUBMB entry for 2.1.1.32
BRENDA references for 2.1.1.32 (Recommended.)
PubMed references for 2.1.1.32
PubMed Central references for 2.1.1.32
Google Scholar references for 2.1.1.32
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Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9: 207–15. PMID 69267419.
Krauss J and Stahelin M (1974). "N2-Guanine specific transfer RNA methyltransferase I from rat liver and leukemic rat spleen". Nucleic Acids Res. 1: 1455–1478.
Krauss J and Stahelin M (1974). "N2-Guanine specific transfer RNA methyltransferase II from rat liver". Nucleic Acids Res. 1: 1479–1496.
Agris PF, Spremulli LL, Brown GM (1974). "tRNA methylases from HeLa cells: purification and properties of an adenine-1-methylase and a guanine-N2-methylase". Arch. Biochem. Biophys. 162: 38–47. PMID 4208689.
Glick JM, Ross S, Leboy PS (1975). "S-adenosylhomocysteine inhibition of three purified tRNA methyltransferases from rat liver". Nucleic. Acids. Res. 2: 1639–51. PMID 1208211.
Izzo P, Gantt R (1977). "Partial purification and characterization of an N2-guanine RNA methyltransferase from chicken embryos". Biochemistry. 16: 3576–81. PMID 19056.
Glick JM, Averyhart VM, Leboy PS (1978). "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta. 518: 158–71. PMID 629973.