tRNA (guanine-N1-)-methyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a tRNA (guanine-N1-)-methyltransferase (EC 2.1.1.31) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + tRNA S-adenosyl-L-homocysteine + tRNA containing N₁-methylguanine

Thus, the two substrates of this enzyme are S-adenosyl methionine and tRNA, whereas its two products are S-adenosylhomocysteine and tRNA containing N1-methylguanine.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:tRNA (guanine-N1-)-methyltransferase. Other names in common use include transfer ribonucleate guanine 1-methyltransferase, tRNA guanine 1-methyltransferase, and S-adenosyl-L-methionine:tRNA (guanine-1-N-)-methyltransferase.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes 1OY5, 1P9P, 1UAJ, 1UAK, 1UAL, and 1UAM.

[edit] References

• IUBMB entry for 2.1.1.31
• BRENDA references for 2.1.1.31 (Recommended.)
• PubMed references for 2.1.1.31
• PubMed Central references for 2.1.1.31
• Google Scholar references for 2.1.1.31
• Hurwitz J, Gold M, Anders M (1964). "The Enzymatic Methylation of Ribonucleic Acid and Deoxyribonucleic Acid. 3. Purification of Soluble Ribonucleic Acid-Methylating Enzymes". J. Biol. Chem. 239: 3462–3473. PMID 14245404. 
• Bjork GR, Svensson I (1969). "Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae". Eur. J. Biochem. 9: 207–215. PMID 69267419. 
• Smolar N, Hellman U, Svensson I (1975). "Two transfer RNA (1-methylguanine) methylases from yeast". Nucleic. Acids. Res. 2: 993–1004. PMID 1096087. 
• Glick JM, Averyhart VM, Leboy PS (1978). "Purification and characterization of two tRNA-(guanine)-methyltransferases from rat liver". Biochim. Biophys. Acta. 518: 158–171. PMID 629973. 

[edit] External links

The CAS registry number for this enzyme class is 37256-99-8.

• IUBMB entry for 2.1.1.31

• KEGG entry for 2.1.1.31

• BRENDA entry for 2.1.1.31

• NiceZyme view of 2.1.1.31

• EC2PDB: PDB structures for 2.1.1.31

• PRIAM entry for 2.1.1.31

• PUMA2 entry for 2.1.1.31

• IntEnz: Integrated Enzyme entry for 2.1.1.31

• MetaCyc entry for 2.1.1.31

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0009019: tRNA (guanine-N1-)-methyltransferase

• AMIGO entry for GO code 0009019: tRNA (guanine-N1-)-methyltransferase