Tolonium chloride
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| Tolonium chloride | |
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| IUPAC name | (7-amino-8-methyl- phenothiazin-3-ylidene)- dimethyl-ammonium |
| Other names | Toluidine blue O |
| Identifiers | |
| CAS number | [92-31-9] |
| PubChem | |
| MeSH | |
| SMILES | CC1=CC2=C(C=C1N)SC3= CC(=[N+](C)C)C=CC3=N2 |
| Properties | |
| Molecular formula | C15H16N3S+ |
| Molar mass | 270.374 g/mol |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
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Tolonium chloride (INN, also known as toluidine blue O) is a compound used in histology.
[edit] Test for lignin
Toluidine blue solution is used in testing for lignin, a complex organic molecule that bonds to cellulose fibres and strengthens and hardens the cell walls in plants. A positive toluidine blue test causes the solution to turn from blue to blue-green. A similar test can be performed with phloroglucinol-HCl solution, which turns red.
[edit] Other Histological Uses
Toluidine Blue is often used to identify Mast cells. It is also used to stain proteoglycans and glycosaminoglycans in tissues such as cartilage.
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