Thymine-DNA glycosylase

From Wikipedia, the free encyclopedia

PDB rendering based on 1wyw.

Available structures: 1wyw, 2d07

Identifiers

Symbol(s)

TDG;

External IDs

OMIM: 601423 HomoloGene: 2415

Gene ontology
Molecular Function:	• damaged DNA binding • protein binding • pyrimidine-specific mismatch base pair DNA N-glycosylase activity • hydrolase activity, acting on glycosyl bonds
Cellular Component:	• nucleus • nucleoplasm
Biological Process:	• base-excision repair • metabolic process

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

n/a

n/a

n/a

Refseq

NM_003211 (mRNA)
NP_003202 (protein)

n/a (mRNA)
n/a (protein)

Location

Chr 12: 102.88 - 102.91 Mb

n/a

Pubmed search

[1]

n/a

Thymine-DNA glycosylase, also known as TDG, is a human gene.^[1]

The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.^[1]

[edit] References

^ ^a ^b Entrez Gene: TDG thymine-DNA glycosylase.

[edit] Further reading

Lindahl T (1982). "DNA repair enzymes.". Annu. Rev. Biochem. 51: 61–87. doi:10.1146/annurev.bi.51.070182.000425. PMID 6287922.
Hardeland U, Bentele M, Lettieri T, et al. (2001). "Thymine DNA glycosylase.". Prog. Nucleic Acid Res. Mol. Biol. 68: 235–53. PMID 11554300.
Chevray PM, Nathans D (1992). "Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun.". Proc. Natl. Acad. Sci. U.S.A. 89 (13): 5789–93. PMID 1631061.
Neddermann P, Jiricny J (1994). "Efficient removal of uracil from G.U mispairs by the mismatch-specific thymine DNA glycosylase from HeLa cells.". Proc. Natl. Acad. Sci. U.S.A. 91 (5): 1642–6. PMID 8127859.
Neddermann P, Jiricny J (1993). "The purification of a mismatch-specific thymine-DNA glycosylase from HeLa cells.". J. Biol. Chem. 268 (28): 21218–24. PMID 8407958.
Neddermann P, Gallinari P, Lettieri T, et al. (1996). "Cloning and expression of human G/T mismatch-specific thymine-DNA glycosylase.". J. Biol. Chem. 271 (22): 12767–74. PMID 8662714.
Sard L, Tornielli S, Gallinari P, et al. (1997). "Chromosomal localizations and molecular analysis of TDG gene-related sequences.". Genomics 44 (2): 222–6. doi:10.1006/geno.1997.4843. PMID 9299239.
Barrett TE, Savva R, Panayotou G, et al. (1998). "Crystal structure of a G:T/U mismatch-specific DNA glycosylase: mismatch recognition by complementary-strand interactions.". Cell 92 (1): 117–29. PMID 9489705.
Missero C, Pirro MT, Simeone S, et al. (2001). "The DNA glycosylase T:G mismatch-specific thymine DNA glycosylase represses thyroid transcription factor-1-activated transcription.". J. Biol. Chem. 276 (36): 33569–75. doi:10.1074/jbc.M104963200. PMID 11438542.
Tini M, Benecke A, Um SJ, et al. (2002). "Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription.". Mol. Cell 9 (2): 265–77. PMID 11864601.
Hardeland U, Steinacher R, Jiricny J, Schär P (2002). "Modification of the human thymine-DNA glycosylase by ubiquitin-like proteins facilitates enzymatic turnover.". EMBO J. 21 (6): 1456–64. doi:10.1093/emboj/21.6.1456. PMID 11889051.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Abu M, Waters TR (2003). "The main role of human thymine-DNA glycosylase is removal of thymine produced by deamination of 5-methylcytosine and not removal of ethenocytosine.". J. Biol. Chem. 278 (10): 8739–44. doi:10.1074/jbc.M211084200. PMID 12493755.
Shimizu Y, Iwai S, Hanaoka F, Sugasawa K (2003). "Xeroderma pigmentosum group C protein interacts physically and functionally with thymine DNA glycosylase.". EMBO J. 22 (1): 164–73. doi:10.1093/emboj/cdg016. PMID 12505994.
Chen D, Lucey MJ, Phoenix F, et al. (2003). "T:G mismatch-specific thymine-DNA glycosylase potentiates transcription of estrogen-regulated genes through direct interaction with estrogen receptor alpha.". J. Biol. Chem. 278 (40): 38586–92. doi:10.1074/jbc.M304286200. PMID 12874288.
Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.". Genomics 83 (1): 153–67. PMID 14667819.
Brandenberger R, Wei H, Zhang S, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation.". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197.
Krześniak M, Butkiewicz D, Samojedny A, et al. (2005). "Polymorphisms in TDG and MGMT genes - epidemiological and functional study in lung cancer patients from Poland.". Ann. Hum. Genet. 68 (Pt 4): 300–12. doi:10.1046/j.1529-8817.2004.00079.x. PMID 15225156.