Spermidine

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Spermidine
IUPAC name N-(3-aminopropyl)butane-1,4-diamine
Other names 1,5,10-triazadecane
Identifiers
CAS number [124-20-9]
PubChem 1102
MeSH Spermidine
SMILES C(CCNCCCN)CN
Properties
Molecular formula C7H19N3
Molar mass 145.246
Density 0.925 g/mL at 25 °C
Melting point

22-25.0 °C

Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)

Infobox disclaimer and references

Spermidine is a polyamine involved in cellular metabolism that can be used to stimulate the enzyme, T7 RNA polymerase, a type of RNA polymerase.

Contents

[edit] Biochemical actions

  • Inhibits neuronal nitric oxide synthase (nNOS).
  • Binds and precipitates DNA.
  • May be used for purification of DNA binding proteins.
  • Stimulates T4 polynucleotide kinase activity.

[edit] Some of the uses

  • Can be used in electroporation while transfering the DNA into the cell under the electrical impulse.

[edit] Safety

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

[edit] See also

[edit] External links