Spermidine
From Wikipedia, the free encyclopedia
| Spermidine | |
|---|---|
| IUPAC name | N-(3-aminopropyl)butane-1,4-diamine |
| Other names | 1,5,10-triazadecane |
| Identifiers | |
| CAS number | [124-20-9] |
| PubChem | |
| MeSH | |
| SMILES | C(CCNCCCN)CN |
| Properties | |
| Molecular formula | C7H19N3 |
| Molar mass | 145.246 |
| Density | 0.925 g/mL at 25 °C |
| Melting point |
22-25.0 °C |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox disclaimer and references |
|
Spermidine is a polyamine involved in cellular metabolism that can be used to stimulate the enzyme, T7 RNA polymerase, a type of RNA polymerase.
Contents |
[edit] Biochemical actions
- Inhibits neuronal nitric oxide synthase (nNOS).
- Binds and precipitates DNA.
- May be used for purification of DNA binding proteins.
- Stimulates T4 polynucleotide kinase activity.
[edit] Some of the uses
- Can be used in electroporation while transfering the DNA into the cell under the electrical impulse.
[edit] Safety
Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

