From Wikipedia, the free encyclopedia
| rncO |
 |
| Type: |
Cis-reg; |
| 2° structure: |
Published; PubMed |
| Seed alignment: |
PubMed |
| Avg length: |
204.1 nucleotides |
| Avg identity: |
80% |
|
The bacterial regulatory element (rncO) is found in the rnc leader sequence. The rnc operon is negatively auto-regulated by transcript stability. rnc, the first gene in the operon codes for RNase III which cleaves the long rncO stem II leading to transcript degradation and a reduction in translation.[1] Matsunaga et al. showed that RNase III cleavage can initiate rnc transcript decay independently of rnc gene translation.[2]
[edit] Structure
Functionally the first 215 nucleotides of the rnc leader have been shown to be sufficient. Within this region three stem-loops were identified. Stem-loop II is cleaved by RNase III, whereas stem-loops I and III are important for stability.[1]
[edit] References
- ^ a b Matsunaga J, Simons EL, Simons RW (1996). "RNase III autoregulation: structure and function of rncO, the posttranscriptional "operator"". RNA 2 (12): 1228–40. PMID 8972772.
- ^ Matsunaga J, Simons EL, Simons RW (1997). "Escherichia coli RNase III (rnc) autoregulation occurs independently of rnc gene translation". Mol. Microbiol. 26 (5): 1125–35. PMID 9426147.
[edit] External links
