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| RNAI |
 |
| Type: |
Gene; antisense; |
| 2° structure: |
Published; PubMed |
| Seed alignment: |
Griffiths-Jones SR |
| Avg length: |
103.5 nucleotides |
| Avg identity: |
71% |
|
RNAI is a non-coding RNA that is an antisense repressor of the replication of some E. coli plasmids, including ColE1. Plasmid replication is usually initiated by RNAII, which acts as a primer by binding to its template DNA. The complementary RNAI binds RNAII prohibiting it from its initiation role. The rate of degradation of RNAI is therefore a major factor in control of plasmid replication. This rate of degradation is aided by the pcnB (plasmid copy number B) gene product[1], which polyadenylates the 3' end of RNAI targeting it for degradation by PNPase.[2]
[edit] References
- ^ He, L; Soderbom F, Wagner EG, Binnie U, Binns N, Masters M (1993). "PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids". Mol Microbiol 9: 1131–1142. doi:10.1111/j.1365-2958.1993.tb01243.x. PMID 7523833.
- ^ Xu, F; Lin-Chao S, Cohen SN (1993). "The Escherichia coli pcnB gene promotes adenylylation of antisense RNAI of ColE1-type plasmids in vivo and degradation of RNAI decay intermediates". Proc Natl Acad Sci U S A 90: 6756–6760. doi:10.1073/pnas.90.14.6756. PMID 7688127.
[edit] External links
