PPP1R8

From Wikipedia, the free encyclopedia

Protein phosphatase 1, regulatory (inhibitor) subunit 8

PDB rendering based on 2jpe.

Available structures: 2jpe

Identifiers

Symbol(s)

PPP1R8; ARD1; ARD-1; NIPP-1; NIPP1; PRO2047

External IDs

OMIM: 602636 MGI: 2140494 HomoloGene: 8555

Gene ontology
Molecular Function:	• magnesium ion binding • DNA binding • RNA binding • endonuclease activity • type 1 serine/threonine specific protein phosphatase inhibitor activity • protein binding • protein phosphatase type 1 regulator activity • ribonuclease E activity • hydrolase activity
Cellular Component:	• nucleus • spliceosome
Biological Process:	• transcription • regulation of transcription, DNA-dependent • mRNA processing • RNA catabolic process • cell proliferation • RNA splicing

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_002713 (mRNA)
NP_002704 (protein)

NM_146154 (mRNA)
NP_666266 (protein)

Location

Chr 1: 28.03 - 28.05 Mb

Chr 4: 132.1 - 132.12 Mb

Pubmed search

[1]

[2]

Protein phosphatase 1, regulatory (inhibitor) subunit 8, also known as PPP1R8, is a human gene.^[1]

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.^[1]

[edit] References

^ ^a ^b Entrez Gene: PPP1R8 protein phosphatase 1, regulatory (inhibitor) subunit 8.

[edit] Further reading

Van Eynde A, Wera S, Beullens M, et al. (1996). "Molecular cloning of NIPP-1, a nuclear inhibitor of protein phosphatase-1, reveals homology with polypeptides involved in RNA processing.". J. Biol. Chem. 270 (47): 28068-74. PMID 7499293.
Wang M, Cohen SN (1994). "ard-1: a human gene that reverses the effects of temperature-sensitive and deletion mutations in the Escherichia coli rne gene and encodes an activity producing RNase E-like cleavages.". Proc. Natl. Acad. Sci. U.S.A. 91 (22): 10591-5. PMID 7524097.
Mishima K, Tsuchiya M, Nightingale MS, et al. (1993). "ARD 1, a 64-kDa guanine nucleotide-binding protein with a carboxyl-terminal ADP-ribosylation factor domain.". J. Biol. Chem. 268 (12): 8801-7. PMID 8473324.
Claverie-Martin F, Wang M, Cohen SN (1997). "ARD-1 cDNA from human cells encodes a site-specific single-strand endoribonuclease that functionally resembles Escherichia coli RNase E.". J. Biol. Chem. 272 (21): 13823-8. PMID 9153239.
Vulsteke V, Beullens M, Waelkens E, et al. (1998). "Properties and phosphorylation sites of baculovirus-expressed nuclear inhibitor of protein phosphatase-1 (NIPP-1).". J. Biol. Chem. 272 (52): 32972-8. PMID 9407077.
Van Eynde A, Pérez-Callejón E, Schoenmakers E, et al. (1999). "Organization and alternate splice products of the gene encoding nuclear inhibitor of protein phosphatase-1 (NIPP-1).". Eur. J. Biochem. 261 (1): 291-300. PMID 10103062.
Jin Q, Beullens M, Jagiello I, et al. (1999). "Mapping of the RNA-binding and endoribonuclease domains of NIPP1, a nuclear targeting subunit of protein phosphatase 1.". Biochem. J. 342 ( Pt 1): 13-9. PMID 10432294.
Chang AC, Sohlberg B, Trinkle-Mulcahy L, et al. (2000). "Alternative splicing regulates the production of ARD-1 endoribonuclease and NIPP-1, an inhibitor of protein phosphatase-1, as isoforms encoded by the same gene.". Gene 240 (1): 45-55. PMID 10564811.
Boudrez A, Beullens M, Groenen P, et al. (2000). "NIPP1-mediated interaction of protein phosphatase-1 with CDC5L, a regulator of pre-mRNA splicing and mitotic entry.". J. Biol. Chem. 275 (33): 25411-7. doi:10.1074/jbc.M001676200. PMID 10827081.
Jagiello I, Van Eynde A, Vulsteke V, et al. (2001). "Nuclear and subnuclear targeting sequences of the protein phosphatase-1 regulator NIPP1.". J. Cell. Sci. 113 Pt 21: 3761-8. PMID 11034904.
Beullens M, Vulsteke V, Van Eynde A, et al. (2001). "The C-terminus of NIPP1 (nuclear inhibitor of protein phosphatase-1) contains a novel binding site for protein phosphatase-1 that is controlled by tyrosine phosphorylation and RNA binding.". Biochem. J. 352 Pt 3: 651-8. PMID 11104670.
Trinkle-Mulcahy L, Sleeman JE, Lamond AI (2002). "Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells.". J. Cell. Sci. 114 (Pt 23): 4219-28. PMID 11739654.
Beullens M, Bollen M (2002). "The protein phosphatase-1 regulator NIPP1 is also a splicing factor involved in a late step of spliceosome assembly.". J. Biol. Chem. 277 (22): 19855-60. doi:10.1074/jbc.M200847200. PMID 11909864.
Boudrez A, Beullens M, Waelkens E, et al. (2002). "Phosphorylation-dependent interaction between the splicing factors SAP155 and NIPP1.". J. Biol. Chem. 277 (35): 31834-41. doi:10.1074/jbc.M204427200. PMID 12105215.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899-903. doi:10.1073/pnas.242603899. PMID 12477932.
Ammosova T, Jerebtsova M, Beullens M, et al. (2003). "Nuclear protein phosphatase-1 regulates HIV-1 transcription.". J. Biol. Chem. 278 (34): 32189-94. doi:10.1074/jbc.M300521200. PMID 12788939.
Jin Q, van Eynde A, Beullens M, et al. (2003). "The protein phosphatase-1 (PP1) regulator, nuclear inhibitor of PP1 (NIPP1), interacts with the polycomb group protein, embryonic ectoderm development (EED), and functions as a transcriptional repressor.". J. Biol. Chem. 278 (33): 30677-85. doi:10.1074/jbc.M302273200. PMID 12788942.
Vulsteke V, Beullens M, Boudrez A, et al. (2004). "Inhibition of spliceosome assembly by the cell cycle-regulated protein kinase MELK and involvement of splicing factor NIPP1.". J. Biol. Chem. 279 (10): 8642-7. doi:10.1074/jbc.M311466200. PMID 14699119.
Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway.". Genome Res. 14 (7): 1324-32. doi:10.1101/gr.2334104. PMID 15231748.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121-7. doi:10.1101/gr.2596504. PMID 15489334.