Image:Ploem 6b.jpg

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[edit] Summary

Fig. 6b: Same tissue and staining as Fig. 6a: Epi-illumination with narrow-band violet excitation light (LP 3mm GG 400 and SP(KP)425 interference filter), a dichroic beam splitter 495nm, reflecting violet light and a barrier filter LP460nm. The filter cube permitted for the first time the observation of blue fluorescent adrenergic nerve fibers, distinctly different from yellow fluorescent mast cells (Ploem, 1971).

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