Pectate lyase

From Wikipedia, the free encyclopedia

In enzymology, a pectate lyase (EC 4.2.2.2) is an enzyme that catalyzes the chemical reaction

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on polysaccharides. The systematic name of this enzyme class is (1->4)-alpha-D-galacturonan lyase. Other names in common use include polygalacturonic transeliminase, pectic acid transeliminase, polygalacturonate lyase, endopectin methyltranseliminase, pectate transeliminase, endogalacturonate transeliminase, pectic acid lyase, pectic lyase, alpha-1,4-D-endopolygalacturonic acid lyase, PGA lyase, PPase-N, endo-alpha-1,4-polygalacturonic acid lyase, polygalacturonic acid lyase, pectin trans-eliminase, and Polygalacturonic acid trans-eliminase. This enzyme participates in pentose and glucuronate interconversions.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 32 structures have been solved for this class of enzymes, with PDB accession codes 1AIR, 1BN8, 1EE6, 1GXM, 1GXN, 1GXO, 1JRG, 1JTA, 1O88, 1O8D, 1O8E, 1O8F, 1O8G, 1O8H, 1O8I, 1O8J, 1O8K, 1O8L, 1O8M, 1OOC, 1PCL, 1PE9, 1PLU, 1R76, 1RU4, 1VBL, 2BSP, 2EWE, 2PEC, 2V8I, 2V8J, and 2V8K.

[edit] References

IUBMB entry for 4.2.2.2
BRENDA references for 4.2.2.2 (Recommended.)
PubMed references for 4.2.2.2
PubMed Central references for 4.2.2.2
Google Scholar references for 4.2.2.2
ALBERSHEIM P, KILLIAS U (1962). "Studies relating to the purification and properties of pectin transeliminase". Arch. Biochem. Biophys. 97: 107–15. doi:10.1016/0003-9861(62)90050-4. PMID 13860094.
EDSTROM RD, PHAFF HJ (1964). "PURIFICATION AND CERTAIN PROPERTIES OF PECTIN TRANS-ELIMINASE FROM ASPERGILLUS FONSECAEUS". J. Biol. Chem. 239: 2403–8. PMID 14235514.
EDSTROM RD, PHAFF HJ (1964). "ELIMINATIVE CLEAVAGE OF PECTIN AND OF OLIGOGALACTURONIDE METHYL ESTERS BY PECTIN TRANS-ELIMINASE". J. Biol. Chem. 239: 2409–15. PMID 14235515.
Nagel CW and Vaughn RH (1961). "The degradation of oligogalacturonides by the polygalacturonase of Bacillus polymyxa". Arch. Biochem. Biophys. 94: 328. doi:10.1016/0003-9861(61)90047-9.
Nasuno S, Starr MP (1967). "Polygalacturonic acid trans-eliminase of Xanthomonas campestris". Biochem. J. 104: 178–85. PMID 6035509.
Pickersgill R, Jenkins J (1997). "Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases". Structure. 5: 677–89. doi:10.1016/S0969-2126(97)00222-0. PMID 9195887.