NFS1

From Wikipedia, the free encyclopedia

NFS1 nitrogen fixation 1 homolog (S. cerevisiae)

Identifiers

NFS1; HUSSY-08; IscS; NIFS

External IDs

OMIM: 603485 MGI: 1316706 HomoloGene: 5463

Gene ontology
Molecular Function:	• transaminase activity • transferase activity • cysteine desulfurase activity
Cellular Component:	• nucleus • mitochondrion • mitochondrial matrix • cytosol
Biological Process:	• sulfur amino acid metabolic process • protein complex assembly • metabolic process

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_181679 (mRNA)
NP_858931 (protein)

NM_010911 (mRNA)
NP_035041 (protein)

Location

Chr 20: 33.72 - 33.75 Mb

Chr 2: 155.82 - 155.84 Mb

Pubmed search

[1]

[2]

NFS1 nitrogen fixation 1 homolog (S. cerevisiae), also known as NFS1, is a human gene.^[1]

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases.^[1]

[edit] References

^ ^a ^b Entrez Gene: NFS1 NFS1 nitrogen fixation 1 homolog (S. cerevisiae).

[edit] Further reading

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. PMID 9373149.
Land T, Rouault TA (1999). "Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to different subcellular compartments is regulated through alternative AUG utilization.". Mol. Cell 2 (6): 807–15. PMID 9885568.
Tong WH, Rouault T (2000). "Distinct iron-sulfur cluster assembly complexes exist in the cytosol and mitochondria of human cells.". EMBO J. 19 (21): 5692–700. doi:10.1093/emboj/19.21.5692. PMID 11060020.
Stanchi F, Bertocco E, Toppo S, et al. (2001). "Characterization of 16 novel human genes showing high similarity to yeast sequences.". Yeast 18 (1): 69–80. doi:10.1002/1097-0061(200101)18:1<69::AID-YEA647>3.0.CO;2-H. PMID 11124703.
Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMID 11256614.
Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20.". Nature 414 (6866): 865–71. doi:10.1038/414865a. PMID 11780052.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Li K, Tong WH, Hughes RM, Rouault TA (2006). "Roles of the mammalian cytosolic cysteine desulfurase, ISCS, and scaffold protein, ISCU, in iron-sulfur cluster assembly.". J. Biol. Chem. 281 (18): 12344–51. doi:10.1074/jbc.M600582200. PMID 16527810.
Biederbick A, Stehling O, Rösser R, et al. (2006). "Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation.". Mol. Cell. Biol. 26 (15): 5675–87. doi:10.1128/MCB.00112-06. PMID 16847322.