mRNA (guanine-N7-)-methyltransferase

From Wikipedia, the free encyclopedia

In enzymology, a mRNA (guanine-N7-)-methyltransferase (EC 2.1.1.56) is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + G(5')pppR-RNA $\rightleftharpoons$ S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA (mRNA containing an N₇-methylguanine cap)

Thus, the two substrates of this enzyme are S-adenosyl methionine and G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppR-RNA.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (guanine-N7-)-methyltransferase. Other names in common use include messenger ribonucleate guanine 7-methyltransferase, guanine-7-methyltransferase, messenger RNA guanine 7-methyltransferase, and S-adenosyl-L-methionine:mRNA (guanine-7-N-)-methyltransferase.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1Z3C and 2HV9.

[edit] References

IUBMB entry for 2.1.1.56
BRENDA references for 2.1.1.56 (Recommended.)
PubMed references for 2.1.1.56
PubMed Central references for 2.1.1.56
Google Scholar references for 2.1.1.56
Ensinger MJ, Martin SA, Paoletti E, Moss B (1975). "Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus". Proc. Natl. Acad. Sci. U. S. A. 72: 2525–9. doi:10.1073/pnas.72.7.2525. PMID 1058472.
Groner Y, Gilboa E, Aviv H (1978). "Methylation and capping of RNA polymerase II primary transcripts by HeLa nuclear homogenates". Biochemistry. 17: 977–82. doi:10.1021/bi00599a005. PMID 629955.
Martin SA, Moss B (1975). "Modification of RNA by mRNA guanylyltransferase and mRNA (guanine-7-)methyltransferase from vaccinia virions". J. Biol. Chem. 250: 9330–5. PMID 1194287.
Martin SA, Paoletti E, Moss B (1975). "Purification of mRNA guanylyltransferase and mRNA (guanine-7-) methyltransferase from vaccinia virions". J. Biol. Chem. 250: 9322–9. PMID 1194286.