Talk:Microsatellite
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[edit] Developing this article
Ive had a bit of an overhaul... I am aware that I still need to sort out references. Hope to do this within the ext few days, unless someone can help! SEJohnston 00:42, 29 April 2006 (UTC)
The article is very vague,there is no mention of how you construct an enriched microsatellite library,i had listed the entire process in the previous edit with specific references for isolation. The standard definition of microsatellites and the classification system has been totally neglected. If you need to edit this page please do so in a manner so as to enable users to gain insight into a specific topic.
- It seems to be less vague than when you last edited it. Can you help improve the article? David D. (Talk) 18:38, 19 May 2006 (UTC)
The article isnt as comprehensive as I have wished it to be, but I do intend to do a lot more work on it... also, there is nothing stopping you from contributing. What are specific parts of the article you feel need to be changed or improved? Criticism is encouraged. Let me know what you want to see added, and I can give it a good try. Hopefully now that my exams and holiday is over, I can really get stuck into it. SEJohnston 12:01, 21 May 2006 (UTC)
- Thanks for asking for desired additions. What role(s) to microsatellites play in the organism — what good are they to the creature? Do microsatellites occur within coding regions (within genes) or within the promoter regions of genes or within exons? Or are they Junk DNA?
- They're "Junk DNA" in the sense that they don't code for anything. I'm not sure about the benefits of satellite DNA. Perhaps because they easily expand the genome they offer possibilities on the formation of beneficial sequences.128.163.224.198 19:13, 27 July 2007 (UTC)
- Satellite DNA says that microsatellites are repeats less than 15 basepairs long, but this says they are 1-4 basepairs in length. Which is correct? Also, that article says that " Microsatellites are often found in transcription units." What does that mean? Thanks again, David.Throop 21:06, 12 December 2006 (UTC)
- In another book it says that microsatellites are 2-5 bps, just to let you know.128.163.224.198 19:14, 27 July 2007 (UTC)
- I've generally heard 2-10bp, with a minisatellite being 10-100or200. A SNP is generally a 1bp polymorphism. —Preceding unsigned comment added by Mkayatta (talk • contribs) 14:04, 2 November 2007 (UTC)
[edit] Difference between Microsatellite and short tandem repeat?
I can't see the difference between "microsatellite" and "short tandem repeat". I would appreciate if anybody wrote it. A good way would include adding "short tandem repeat" somewhere in the neat assortment on Repeated sequence (DNA). Thank you. Mortsggah 16:38, 6 April 2007 (UTC)
They are basically the same thing.
Well, then I'll propose a merge between the articles. Mortsggah 14:24, 17 April 2007 (UTC)
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- Yes, they should be merged. According to the book I'm reading (Molecular Evolution: A Phylogenetic Approach) the two things are one and the same.128.163.224.198 19:17, 27 July 2007 (UTC)
In agreement here, SSRs (microsatellites) and STRs are in essence the same thing and could be covered under the same Wiki topic, both are co-dominant in nature and have similar functionality12.210.165.57 03:46, 8 August 2007 (UTC)Matt
Sounds like a fine idea to me! Coming from a forensic science background, we never call them minisats which is why I never even thought to look if there was such an article already in existence. But it's just another way to say the same thing, so a merge definitely makes sense. Sekiyu 04:22, 22 August 2007 (UTC)
Specifically, a microsatellite (as opposed to minisatellite) is a repetition of 1 to 6 nucleotides. The term short tandem repeat seems more vague. I think you guys should just remove the short tandem repeat article and move its contents to the microsatellite article. Genericforms 20:08, 19 September 2007 (UTC)
i was told that SSRs (micro-satellites) are a 1-5 nucleotide repeat structure, STRs are 5-10, and VNTRs are 10-100. obviously there is a bit of overlap in there, but they are definitely different in their frequency throughout the different genomes. larger repeats (STR and VNTR) seem to be used more heavily in human research, while the smaller repeats (SSRs) are used extensively in plant research. Dave C. —Preceding unsigned comment added by 69.233.131.125 (talk) 07:11, 2 October 2007 (UTC)
SSRS are not exactly microsatellite; actually they are composed of two types micrsatellite (1-5 kbp long, they are composed of 20-50 tandem repeat units and are found in centromere and telomeres) and minisatellites (can be up to 600 bp and are composed of tandem repeat units that are typically 1-4 bp in lenth). Minisatellites are sometimes found in transcriptional units. —Preceding unsigned comment added by TAKEN00 (talk • contribs) 22:54, 11 December 2007 (UTC)
[edit] Merge Discussion First
Discussions on merging between STR and microsatellites held at two places, Talk:Microsatellite(HERE) and Talk:Short tandem repeat. So we should merge discussions at first :) Also, we should discuss based on literatures, since there seems wide variety of what is microsatellite and what is STR. I have already picked up some literatures at Talk:Short tandem repeat. Please check it out.--Mzaki 06:19, 12 October 2007 (UTC)
