Methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase

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In enzymology, a methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (EC 2.1.1.74) is an enzyme that catalyzes the chemical reaction

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH₂ $\rightleftharpoons$ tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD

The 3 substrates of this enzyme are 5,10-methylenetetrahydrofolate, tRNA containing uridine at position 54, and FADH2, whereas its 3 products are tetrahydrofolate, tRNA containing ribothymidine at position 54, and FAD.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is 5,10-methylenetetrahydrofolate:tRNA (uracil-5-)-methyl-transferase. Other names in common use include (FADH2-oxidizing), folate-dependent ribothymidyl synthase, methylenetetrahydrofolate-transfer ribonucleate uracil, 5-methyltransferase, 5,10-methylenetetrahydrofolate:tRNA-UPsiC, and (uracil-5-)-methyl-transferase.

[edit] References

IUBMB entry for 2.1.1.74
BRENDA references for 2.1.1.74 (Recommended.)
PubMed references for 2.1.1.74
PubMed Central references for 2.1.1.74
Google Scholar references for 2.1.1.74
Delk AS, Nagle DP Jr, Rabinowitz JC (1980). "Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2". J. Biol. Chem. 255: 4387–90. PMID 6768721.
Becker HF, Motorin Y, Sissler M, Florentz C, Grosjean H (1997). "Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the T psi-loop of yeast tRNAs". J. Mol. Biol. 274: 505–18. doi:10.1006/jmbi.1997.1417. PMID 9417931.