Lieberman-Burchard test

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The Lieberman-Burchard or acetic anhydride test is used for the determination of cholesterol. The formation of a green or green-blue color after a few minutes is positive.

Lieberman-Burchard is a reagent used in a colorimetric test to detect cholesterol, which gives a deep green color. This color begins as a purplish, pink color and progresses through to a light green then very dark green color. The color is due to the hydroxyl group (-OH) of cholesterol reacting with the reagents and increasing the conjugation of the un-saturation in the adjacent fused ring. this test uses acetic anhydride and sulfuric acid as reagents and caution must be exercised so as not to receive severe burns from the reagents.