LGMN

From Wikipedia, the free encyclopedia

Legumain

Identifiers

LGMN; AEP; LGMN1; PRSC1

External IDs

OMIM: 602620 MGI: 1330838 HomoloGene: 38075

Gene ontology
Molecular Function:	• legumain activity
Cellular Component:	• lysosome • late endosome • apical part of cell
Biological Process:	• proteolysis • negative regulation of body size

RNA expression pattern

More reference expression data

Orthologs

Human

Mouse

Refseq

NM_001008530 (mRNA)
NP_001008530 (protein)

NM_011175 (mRNA)
NP_035305 (protein)

Location

Chr 14: 92.24 - 92.28 Mb

Chr 12: 102.8 - 102.84 Mb

Pubmed search

[1]

[2]

Legumain, also known as LGMN, is a human gene.^[1]

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.^[1]

[edit] References

^ ^a ^b Entrez Gene: LGMN legumain.

[edit] Further reading

Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. PMID 8125298.
Tanaka T, Inazawa J, Nakamura Y (1996). "Molecular cloning of a human cDNA encoding putative cysteine protease (PRSC1) and its chromosome assignment to 14q32.1.". Cytogenet. Cell Genet. 74 (1-2): 120–3. PMID 8893817.
Chen JM, Dando PM, Rawlings ND, et al. (1997). "Cloning, isolation, and characterization of mammalian legumain, an asparaginyl endopeptidase.". J. Biol. Chem. 272 (12): 8090–8. PMID 9065484.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. PMID 9373149.
Chen JM, Dando PM, Stevens RA, et al. (1998). "Cloning and expression of mouse legumain, a lysosomal endopeptidase.". Biochem. J. 335 ( Pt 1): 111–7. PMID 9742219.
Halfon S, Patel S, Vega F, et al. (1998). "Autocatalytic activation of human legumain at aspartic acid residues.". FEBS Lett. 438 (1-2): 114–8. PMID 9821970.
Manoury B, Hewitt EW, Morrice N, et al. (1999). "An asparaginyl endopeptidase processes a microbial antigen for class II MHC presentation.". Nature 396 (6712): 695–9. doi:10.1038/25379. PMID 9872320.
Chen JM, Rawlings ND, Stevens RA, Barrett AJ (1999). "Identification of the active site of legumain links it to caspases, clostripain and gingipains in a new clan of cysteine endopeptidases.". FEBS Lett. 441 (3): 361–5. PMID 9891971.
Chen JM, Fortunato M, Barrett AJ (2001). "Activation of human prolegumain by cleavage at a C-terminal asparagine residue.". Biochem. J. 352 Pt 2: 327–34. PMID 11085925.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMID 12477932.
Li DN, Matthews SP, Antoniou AN, et al. (2003). "Multistep autoactivation of asparaginyl endopeptidase in vitro and in vivo.". J. Biol. Chem. 278 (40): 38980–90. doi:10.1074/jbc.M305930200. PMID 12860980.
Burster T, Beck A, Tolosa E, et al. (2004). "Cathepsin G, and not the asparagine-specific endoprotease, controls the processing of myelin basic protein in lysosomes from human B lymphocytes.". J. Immunol. 172 (9): 5495–503. PMID 15100291.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMID 15489334.
Murthy RV, Arbman G, Gao J, et al. (2005). "Legumain expression in relation to clinicopathologic and biological variables in colorectal cancer.". Clin. Cancer Res. 11 (6): 2293–9. doi:10.1158/1078-0432.CCR-04-1642. PMID 15788679.
Oh JH, Yang JO, Hahn Y, et al. (2006). "Transcriptome analysis of human gastric cancer.". Mamm. Genome 16 (12): 942–54. doi:10.1007/s00335-005-0075-2. PMID 16341674.