Isopenicillin-N N-acyltransferase

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In enzymology, an isopenicillin-N N-acyltransferase (EC 2.3.1.164) is an enzyme that catalyzes the chemical reaction

phenylacetyl-CoA + isopenicillin N + H₂O $\rightleftharpoons$ CoA + penicillin G + L-2-aminohexanedioate

The 3 substrates of this enzyme are phenylacetyl-CoA, isopenicillin N, and H₂O, whereas its 3 products are CoA, penicillin G, and L-2-aminohexanedioate.

This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:isopenicillin N N-acyltransferase. Other names in common use include acyl-coenzyme A:isopenicillin N acyltransferase, and isopenicillin N:acyl-CoA: acyltransferase. This enzyme participates in penicillin and cephalosporin biosynthesis.

[edit] References

IUBMB entry for 2.3.1.164
BRENDA references for 2.3.1.164 (Recommended.)
PubMed references for 2.3.1.164
PubMed Central references for 2.3.1.164
Google Scholar references for 2.3.1.164
Tobin MB, Fleming MD, Skatrud PL, Miller JR (1990). "Molecular characterization of the acyl-coenzyme A:isopenicillin N acyltransferase gene (penDE) from Penicillium chrysogenum and Aspergillus nidulans and activity of recombinant enzyme in Escherichia coli". J. Bacteriol. 172: 5908–14. PMID 2120195.
Aplin RT, Baldwin JE, Roach PL, Robinson CV, Schofield CJ (Pt 2). "Investigations into the post-translational modification and mechanism of isopenicillin N:acyl-CoA acyltransferase using electrospray mass spectrometry". Biochem. J. 294: 357–63. PMID 8396910.