Iodoacetate
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The iodoacetate anion is an alkylating agent which acts on cysteine residues in proteins. It is often used to modify SH-groups to prevent the re-formation of disulfide bonds after the reduction of cystine residues to cysteine during protein sequencing.
The protonated form of iodoacetate is often called monoiodoacetic acid or simply iodoacetic acid.
[edit] See also
[edit] References
- A. K. Knap and R. F. Pratt (1991). "Inactivation of the RTEM-1 cysteine beta-lactamase by iodoacetate. The nature of active-site functional groups and comparisons with the native enzyme". Biochem. J. 273: 85–91.
- Dickens F (1933). "Interaction of halogenacetates and SH compounds. The reaction of halogenacetic acids with glutathione and cysteine. The mechanism of iodoacetate poisoning of glyoxalase.". Biochem. J. 27: 1141–1151. PMID 16745202.
