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Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets fused to the N-terminal of the gene.
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AAAsBiophysics 20:29, 17 July 2007 (UTC)
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| Date/Time | Dimensions | User | Comment | |
|---|---|---|---|---|
| current | 19:18, 17 July 2007 | 773×607 (30 KB) | Jfhaller (Talk | contribs) | (Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets f) |
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