Image:His-tag-primers.png
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Example of primer designed to add a 6xHis-tag using PCR. Eighteen bases coding six histidines are inserted right after the STAR codon or right before the STOP codon. At least 16 bases specific of a gene of interest are need it next to the His-tag.
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AAAsBiophysics 20:27, 17 July 2007 (UTC)
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| Date/Time | Dimensions | User | Comment | |
|---|---|---|---|---|
| current | 19:38, 17 July 2007 | 565×106 (8 KB) | Jfhaller (Talk | contribs) | (Example of primer designed to add a 6xHis-tag using PCR. Eighteen bases coding six histidines are inserted right after the STAR codon or right before the STOP codon. At least 16 bases specific of a gene of interest are need it next to the His-tag. ) |
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