Dolichyl-diphosphooligosaccharide-protein glycotransferase

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In enzymology, a dolichyl-diphosphooligosaccharide-protein glycotransferase (EC 2.4.1.119) is an enzyme that catalyzes the chemical reaction

dolichyl diphosphooligosaccharide + protein L-asparagine dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine

Thus, the two substrates of this enzyme are dolichyl diphosphooligosaccharide and protein L-asparagine, whereas its 3 products are dolichyl diphosphate, glycoprotein with the oligosaccharide chain attached by N-glycosyl, and linkage to protein L-asparagine.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is dolichyl-diphosphooligosaccharide:protein-L-asparagine oligopolysaccharidotransferase. Other names in common use include dolichyldiphosphooligosaccharide-protein glycosyltransferase, asparagine N-glycosyltransferase, dolichyldiphosphooligosaccharide-protein oligosaccharyltransferase, dolichylpyrophosphodiacetylchitobiose-protein glycosyltransferase, oligomannosyltransferase, oligosaccharide transferase, dolichyldiphosphoryloligosaccharide-protein, and oligosaccharyltransferase. This enzyme participates in n-glycan biosynthesis and glycan structures - biosynthesis 1.

Contents 1 Structural studies 2 References 3 External links 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code 1RKL.

[edit] References

• IUBMB entry for 2.4.1.119
• BRENDA references for 2.4.1.119 (Recommended.)
• PubMed references for 2.4.1.119
• PubMed Central references for 2.4.1.119
• Google Scholar references for 2.4.1.119
• Das RC, Heath EC (1980). "Dolichyldiphosphoryloligosaccharide--protein oligosaccharyltransferase; solubilization, purification, and properties". Proc. Natl. Acad. Sci. U. S. A. 77: 3811–5. doi:10.1073/pnas.77.7.3811. PMID 6933437. 

[edit] External links

The CAS registry number for this enzyme class is 75302-32-8.

• IUBMB entry for 2.4.1.119

• KEGG entry for 2.4.1.119

• BRENDA entry for 2.4.1.119

• NiceZyme view of 2.4.1.119

• EC2PDB: PDB structures for 2.4.1.119

• PRIAM entry for 2.4.1.119

• PUMA2 entry for 2.4.1.119

• IntEnz: Integrated Enzyme entry for 2.4.1.119

• MetaCyc entry for 2.4.1.119

• Atomic-resolution structures of enzymes belonging to this class

[edit] Gene Ontology (GO) codes

• EGO entry for GO code 0004579: dolichyl-diphosphooligosaccharide-protein glycotransferase

• AMIGO entry for GO code 0004579: dolichyl-diphosphooligosaccharide-protein glycotransferase