Chorismate synthase

From Wikipedia, the free encyclopedia

In enzymology, a chorismate synthase (EC 4.2.3.5) is an enzyme that catalyzes the chemical reaction

5-O-(1-carboxyvinyl)-3-phosphoshikimate $\rightleftharpoons$ chorismate + phosphate

Hence, this enzyme has one substrate, 5-O-(1-carboxyvinyl)-3-phosphoshikimate, and two products, chorismate and phosphate.

This enzyme belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The systematic name of this enzyme class is 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase (chorismate-forming). This enzyme is also called 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase. This enzyme participates in phenylalanine, tyrosine and tryptophan biosynthesis.

1 Structural studies
2 References
3 External links
- 3.1 Gene Ontology (GO) codes

[edit] Structural studies

As of late 2007, 9 structures have been solved for this class of enzymes, with PDB accession codes 1Q1L, 1QXO, 1R52, 1R53, 1SQ1, 1UM0, 1UMF, 1ZTB, and 2G85.

[edit] References

IUBMB entry for 4.2.3.5
BRENDA references for 4.2.3.5 (Recommended.)
PubMed references for 4.2.3.5
PubMed Central references for 4.2.3.5
Google Scholar references for 4.2.3.5
Gaertner FH, Cole KW (1973). "Properties of chorismate synthase in Neurospora crassa". J. Biol. Chem. 248: 4602–9. PMID 4146266.
Morell H, Clark MJ, Knowles PF, Sprinson DB (1967). "The enzymic synthesis of chorismic and prephenic acids from 3-enolpyruvylshikimic acid 5-phosphate". J. Biol. Chem. 242: 82–90. PMID 4289188.
Welch GR, Cole KW, Gaertner FH (1974). "Chorismate synthase of Neurospora crassa: a flavoprotein". Arch. Biochem. Biophys. 165: 505–18. doi:10.1016/0003-9861(74)90276-8. PMID 4155270.
Bornemann S, Lowe DJ, Thorneley RN (1996). "The transient kinetics of Escherichia coli chorismate synthase: substrate consumption, product formation, phosphate dissociation, and characterization of a flavin intermediate". Biochemistry. 35: 9907–16. doi:10.1021/bi952958q. PMID 8703965.
Bornemann S, Theoclitou ME, Brune M, Webb MR, Thorneley RN, Abell C (2000). "A Secondary beta Deuterium Kinetic Isotope Effect in the Chorismate Synthase Reaction". BIO-ORGANIC. CHEMISTRY. 28: 191–204. PMID 11034781.
Osborne A, Thorneley RN, Abell C, Bornemann S (2000). "Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction". J. Biol. Chem. 275: 35825–30. doi:10.1074/jbc.M005796200. PMID 10956653.