Chelex 100

From Wikipedia, the free encyclopedia

Chelex 100 is a chelating resin used to purify other compounds via ion exchange. It is noteworthy for its ability to bind transition metal ions.

It is a styrene-divinylbenzene resin containing iminodiacetic acid groups.

A concentrated solution of metals is obtained by eluting the resin with a small volume of 2 M nitric acid, which protonates the iminodiacetate groups.

Chelex resin is often used by DNA extraction. Polar resin beads binds polar cellular components after breaking open cells. Non-polar nuclear DNA and RNA remain in water solution above chelex.

[edit] References

• Daniel Harris. Quantitative Chemical Analysis, seventh edition, 2007. ISBN: 0-7167-7041-5. Page 594.
• R. N. Ceo, M. R. Kazerouni, and K. Rengan. "Sorption of silver ions by Chelex 100 chelating resin." Journal of Radionanalytical and Nuclear Chemistry, 1992. Abstract.