Talk:Cell culture

From Wikipedia, the free encyclopedia

Molecular and Cellular Biology WikiProject
MCB Portal
This article is within the scope of the Molecular and Cellular Biology WikiProject. To participate, visit the WikiProject for more information. The WikiProject's current monthly collaboration is focused on improving Restriction enzyme.
B This article has been rated as B-Class on the assessment scale.
Top This article is on a subject of Top-importance within molecular and cellular biology.

Article Grading: The article has been rated for quality and/or importance but has no comments yet. If appropriate, please review the article and then leave comments here to identify the strengths and weaknesses of the article and what work it will need.
To-do list for Cell culture: edit · history · watch · refresh

Here are some tasks you can do:

    I'm giving this article some attention, but welcome anyone else who would like to work on expanding it. Courtland 02:25, 2005 Mar 13 (UTC)

    Contents

    [edit] Article organization

    current thought on organizing the article:

    • Introduction (present)
    • Key concepts (begun)
    • Uses of cell culture
    • Specific examples
    • Notes & references (begun)
    • External links to methods and manuals
    • Related topics

    I'm thinking that all types of cell culture could be addressed in a general sense, with specific articles spinning off for things like insect cell culture, plant tissue culture, culture of anaerobic bacterial pathogens, viral culture, etc. Courtland 02:29, 2005 Mar 13 (UTC)


    I don't think that there needs to be much mention of microbial culturing. Make a link to microbiology, fermentation etc. In my experience cell culture in it's modern sense means, to the laboratory scientist, mainly animal and plant cell culture. Microbial cell culture is a term one hardly ever hears.--Alun 06:04, 11 May 2005 (UTC)

    [edit] Related articles (etc.) to consider while writing

    (not articles yet)

    [edit] image requests

    some might/should go into related articles Courtland 02:50, 2005 Mar 13 (UTC)

    		 It is requested that an image of large scale bacteria or yeast liquid culture bacterial petri culture, mammalian plate culture, basic materials required to prepare agar plates, basic tools required for bacterial culture, basic tools required for mammalian culture be included in this article to improve its quality, if possible.
    Two images have so far been provided. LostLucidity (talk) 19:08, 12 March 2008 (UTC)

    [edit] Morpholino

    Sorry for my ignorance, but what is the relevance of this section to "cell culture"? Kaisershatner 18:32, 18 April 2006 (UTC)

    "Preclinical research with Morpholino antisense oligos has shown efficacy of the antisense against influenza in cell cultures. Cultures of African green monkey kidney cells (vero cells) were pretreated with Morpholino antisense oligos conjugated with arginine-rich peptides to enhance penetration of the oligos into the cytosol. Targeting translation-blocking Morpholinos against the nucleoprotein 1 mRNA or one of the polymerase protein mRNAs caused 2-3 log10 reductions in influenzavirus titer three days post-infection. When Morpholinos were administered post-infection, less antiviral activity was measured. [1]
    AVI BioPharma reports that when tested against several influenza strains by several independent laboratories, Morpholino oligos have suppressed viral replication and in one cse both replication and transcription were repressed. Co-administration of several Morpholino sequences caused as much as eightfold improvement in antiviral activity. [2]
    Can you explain why it doesn't belong in cell culture? Don't the two paragraphs talk about cell culture? If you are saying other stuff is more directly relevant, then my response is, well add that more directly relevent stuff and then the less directly relevant stuff can be removed at that time. If you are saying, no it really isn't even indirectly related, then you must know more about this than I do (which wouldn't be hard, I know very little about it); in which case we just keep it removed. WAS 4.250 19:31, 18 April 2006 (UTC)

    [edit] op.cit

    Shouldn't "op.cit" contain more information? WAS 4.250 19:23, 18 April 2006 (UTC)

    [edit] Cell culture and Tissue culture fusion

    For something that has so many applications in agriculture, medicine, and basic research both articles are extremely weak. The tissue culture should have the history and animal-plant distinctions and organ,tissue, cell distinction. I can see the cell culture needs to be its own article but is it at that stage presently or should it be fused with the weaker Tissue culture? The history of tissue culture could be a distinct article with the history of methodologies in culture, synchronizing cells, labeling cells, heterokaryons, hybridomas, suspension, substrates, transformation, contact inhibition, Hayflick senescence and now telomeres, SV-40 virus, etc. GetAgrippa 19:05, 26 January 2007 (UTC)

    [edit] Tempted to vandalise

    I am tempted to add at the top:

    For those dots in your cells when stained, see mycoplasma.

    Maybe on 1 april next year... --Squidonius (talk) 15:21, 9 May 2008 (UTC)

    [edit] Insect cell culture

    Culture of non-mammalian cells needs a section on insect cell culture. I'm not an expert, and was actually looking to see if there was anything here on the topic. In my very limited understanding, I gather that people sometimes use insect cells in applications when mammalian cells might introduce unwanted background (because there is so much difference between insects and mammals). Can someone else comment or make an appropriate contribution to the article? Lcwilsie (talk) 16:09, 9 May 2008 (UTC)

    [edit] cell line lists

    There are two lists of common cell lines at the end of this article. Perhaps they should be combined into one? 142.103.207.10 (talk) 22:31, 14 May 2008 (UTC)

    Absolutely. I like the table format, in that it is sort-able. However, since the table isn't comprehensive (and would be ridiculously long if it were), does the list add any value? How do we decide which cell lines to include? Is there an external link somewhere with a comprehensive list? Should there simply be some representative examples cited in the text (such as, "Cell lines have been generated from many tissues and species such as _______")? Lcwilsie (talk) 13:31, 21 May 2008 (UTC)
    partially Concur I added 2 lines as it took me 2 hours to find out what they actually stand for (Unfortuantelly it is nice trivial information that is getting lost). People that check the Methods pages on wikipedia tend to be people in research that are looking up facts like "how does it work" or what does that stand for. So I would opt to merge and keep the table, maybe on a separte page so less offensive to the eye? although the morphology section may be incorrect in terminology but I cannot find the right ref.--Squidonius (talk) 21:13, 21 May 2008 (UTC)