CEDARLANE

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Cedarlane Laboratories Ltd.
Type	Privately held company
Founded	1957 by Richard Course
Headquarters	Burlington, Ontario, Canada
Industry	Biotech
Products	Lympholyte, CELLECT, Research Reagents
Employees	75 (2008)
Website	www.cedarlanelabs.com

Cedarlane Laboratories Ltd. is both a manufacturer and a distributor in the Biotech industry. The company was incorporated in 1975 to become both ISO 9001:2000 and ISO 13485:2003 certified. Manufactured products include monoclonal antibodies, polyclonal antibodies, cell separation media, complement for tissue typing, and immunocolumns^[1]. Growing as a manufacturer, Cedarlane has also evolved to become one of the largest reagent distribution companies in Canada, with many international ties throughout the world. Growing from a small group cancer researchers operating out of Hornby, Ontario, Cedarlane now operates its Canadian office out of Burlington, Ontario. In 2006, Cedarlane opened a branch office in Burlington, North Carolina in order to better provide researchers in the United States with the reagents that they require^[2]. The company is well known throughout the scientific community as a source for research needs with a focus on the advancement of life science research. With more than 700 suppliers and international sales to over 25 countries, Cedarlane has grown to become a global leader in life science reagent distribution^[3].

[edit] History

Cedarlane was started by Richard Course in 1957 as a home-based manufacturer of complement. In 1975, Dr. S. Abrahams and Dr. A. J. Farmilo joined Richard and Cedarlane was incorporated. Richard Course was previously with the Ontario Cancer Institute and Dr. Abrahams and Dr. Farmilo were recent graduates of the University of Toronto. Ownership changed hands in 1986, when Cedarlane was purchased by John Course and Cynthia Greer. John Course had been working in the company’s production department since 1975 and Cynthia Greer worked in the research and development department since 1982. Operating in Southern Ontario, Cedarlane started by focusing its production on immunology research-based products, eventually integrating a distribution role into its corporate environment. Both the founders and current owners have played key roles in Cedarlane's growth and success over the past 50 years.

[edit] Lympholyte® - cell separation media

Lympholyte® is a cell separation media developed by Cedarlane Laboratories Ltd. for the purpose of isolating lymphocytes from the lymphoid organs or blood of a particular species. Cedarlane produces Lympholyte® specific for mouse, rat, rabbit and human lymphocytes which allows for a wide range of cells to be applied, making a historically uncertain task much more stablized. For the isolation of cells whose density falls outside the range of the ‘premade’ Lympholyte media, there is Lympholyte®-1.1, a density gradient medium whose density can be set where needed.

Lympholyte® – H is a density gradient separation medium specifically designed for the isolation of viable lymphocytes and monocytes from human peripheral and cord blood. Lympholyte®-H can be utilized with a simple protocol for the elimination of erythrocytes and dead cells from human blood. Lympholyte®-H also removes the majority of granulocytes (including neutrophils). The resulting cell population consists of a high and non-selective recovery of viable human lymphocytes and monocytes.

Lympholyte®-1.1 is a density separation medium specifically designed for the isolation of islet cells from the pancreas. Lympholyte®-1.1 can be used undiluted to eliminate non-islet tissue such as exocrine fragments, lymph nodes, ganglia, ductal and vascular tissue from pancreatic cell suspension. The resulting cell population consists mainly of islet cells that may be expanded for use in vivo.

Lympholyte®-M is a density separation medium specifically designed for the isolation of viable lymphocytes from murine lymphoid cell suspensions. Lympholyte®-M can be utilized with a simple protocol to eliminate erythrocytes, dead cells and debris from murine spleen, lymph node, thymus and bone marrow suspensions. The resulting cell population demonstrates a high and non-selective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity, FACS assays, and in in vivo and in vitro functional studies. Other successful applications include: i) the removal of dead cells in sequential cytotoxicity studies eg. B-cell depletion. ii) the removal of erythrocytes, dead cells and debris from other murine tissue suspensions including liver and lung. iii) the harvesting of viable cells and removal of dead cells and debris from various clone cell and hybridoma cell lines. iv) the isolation of murine nuclear epidermal cells.

Lympholyte®-Mammal is a density separation medium specifically designed for the isolation of viable lymphocytes and monocytes from the peripheral blood of most mammalian species. It consists of Sodium Diatrizoate combined with Dextran to induce erythrocyte aggregation and reduce platelet aggregation resulting in a higher yield of lymphocytes and monocytes. Lympholyte®-Mammal can be utilized with a simple protocol for the elimination of erythrocytes and dead cells from the blood of most mammalian species. Lympholyte®-Mammal also removes the majority of granulocytes (including neutrophils). The resulting cell population demonstrates a high and non-selective recovery of viable lymphocytes and monocytes.

Lympholyte®-poly is a ready-made sterile and endotoxin tested solution which is a mixture of sodium metrizoate and Dextran 500 that allows for the isolation of human polymorphonuclear granulocytes from whole blood. Lympholyte®-poly is ideally suited for isolation of polymorphonuclear granulocytes (neutrophils, eosinophils) from human whole blood. Mononuclear and the polymorphonuclear leukocytes are separated into two distinct bands free from erythrocytes. It has also been found to be suitable for the isolation of neutrophils from bronchial lung lavage suspension.

Lympholyte®-Rabbit is a density separation medium specifically designed for the isolation of viable lymphocytes from rabbit lymphoid cell suspensions. Lympholyte®-Rabbit can be utilized with a simple protocol for the elimination of erythrocytes, dead cells and debris from rabbit spleen, lymph node and thymus suspensions. The resulting cell population demonstrates a high and nonselective recovery of viable lymphocytes that are suitable for use as target cells in cytotoxicity and FACS assays, as well as in in vivo and in vitro functional studies. Other successful applications include: i) the removal of dead cells in sequential cytotoxicity studies eg. B-cell depletion. ii) the removal of erythrocytes, dead cells and debris from other rabbit tissue suspensions including bone marrow, liver and lung.

Lympholyte®-Rat is a density separation medium specifically designed for the isolation of viable lymphocytes from rat lymphoid cell suspensions. Lympholyte®-Rat can be utilized with a simple protocol for the elimination of erythrocytes, dead cells and debris from rat spleen, lymph node, thymus and bone marrow suspensions. The resulting cell population demonstrates a high and non-selective recovery of viable lymphocytes, which are suitable for use as target cells in cytotoxicity and FACS assays, as well as in in vivo and in vitro functional studies. Other successful applications include: i) the removal of dead cells in sequential cytotoxicity studies eg. B-cell depletion. ii) the removal of erythrocytes, dead cells and debris from other rat tissue suspensions including liver and lung. iii) the harvesting

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